The rapid identification of European Armillaria species from soil samples by nested PCR

LOCHMAN, Jan, Omar ŠERÝ and Vladimír MIKEŠ. The rapid identification of European Armillaria species from soil samples by nested PCR. FEMS Microbiology Letters. Amsterdam, Netherlands: Elsevier Science B. V., 2004, vol. 2004, No 237, p. 105-110. ISSN 0378-1097.

Basic information

Original name

The rapid identification of European Armillaria species from soil samples by nested PCR

Name in Czech

Rychlá identifikace evropských druhů václavek z půdních vzorků pomocí metody nested PCR

Authors

LOCHMAN, Jan (203 Czech Republic), Omar ŠERÝ (203 Czech Republic) and Vladimír MIKEŠ (203 Czech Republic, guarantor)

Edition

FEMS Microbiology Letters, Amsterdam, Netherlands, Elsevier Science B. V. 2004, 0378-1097

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.840

RIV identification code

RIV/00216224:14310/04:00010230

Organization unit

Faculty of Science

UT WoS

000223031300014

Keywords in English

Armillaria; PCR; soil

Tags

Armillaria, PCR, soil

Tags

International impact, Reviewed

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Abstract

V originále

New specific primers AR1 and AR2 were successfully used for the amplification of a specific part of internal transcribed spacer (ITS) of rDNA of Armillaria isolated from soil samples.The individual species were distinguished by restriction fragment length polymorphisms (RFLP) analysis with restriction endonucleases HinfI. For the first time, a method is described enabling the identification of Armillaria species within one day directly from soil samples without the need for previous isolation and cultivation of mycelium

In Czech

V rámci studie byly připraveny specifické primery AR1 a AR2, které slouží k amplifikaci specifické části oblasti ITS v rDNA václavek. Různé druhy václavek byly rozlišovány pomocí RFLP amplifikovaného fragmentu. Námi vyvinutá metoda umožňuje v průběhu jednoho dne určit druh václavky z půdního vzorku pomocí nested PCR a to bez zdlouhavé kultivace.

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Links

MSM 143100005, plan (intention)

Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism