Detailed Information on Publication Record
2004
Visualization of high light effects on intrathalline variability of photosynthetic processes in hydrated Lasallia pustulata
HÁJEK, Josef and Miloš BARTÁKBasic information
Original name
Visualization of high light effects on intrathalline variability of photosynthetic processes in hydrated Lasallia pustulata
Name in Czech
Vizualizace účinku vysokých hodnot ozářenosti na vnitrostélkovou variabilitu fotosyntetických procesů v ovlhčených stélkách lišejníku Lasallia pustulata
Authors
HÁJEK, Josef (203 Czech Republic, guarantor) and Miloš BARTÁK (203 Czech Republic)
Edition
Tartu, Estonia, Book of Abstracts. 5th IAL Symposium, p. 45-45, 2004
Publisher
University of Tartu
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Estonia
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/04:00019780
Organization unit
Faculty of Science
ISBN
9985-56-922-9
Keywords in English
lichen; chlorophyll fluorescence imaging; stress physiology
Změněno: 31/5/2006 17:44, doc. Mgr. Josef Hájek, Ph.D.
V originále
It is expected that hydrated lichen thalli are more susceptible to negative effects of high light (HL) under low rather then optimum temperature of thallus. In our experiments, we investigated response of foliose lichen species (Lasallia pustulata) to HL stress at low temperature under laboratory conditions. Hydrated thalli were exposed to HL of 500 mmol (CO2) m-2 s-1 for 30 min under 5 oC. Response of photosynthetic parameters (Fv/Fm - potential yield of PS II photochemistry, FII - quantum yield of PS II photochemistry, qN - non-photochemical quenching, qT+I - photoinhibition-related quenching) was monitored during HL treatment and subsequent 6 h recovery in dark using chlorophyll (Chl) fluorescence imaging technique False color images of lichen thallus showing heterogeneity of the above-specified Chl fluorescence parameters over the thalli were taken using a kinetic fluorometric CCD Camera (FluorCam FL-400, PSI, Czech Republic). Subsequent image analysis allowed us to specify zones and anatomic structures exhibiting different values of Chl fluorescence parameters. When considering whole lichen thallus, HL treatment led to 23 and 20 % decrease in Fv/Fm and FII, respectively. Dark recovery led to a gradual increase in both parameters. Fast phase of recovery, attributed to regulatory and protective mechanisms, was finished after 90 min recovery in dark. Slow phase of recovery, attributed to reparatory mechanisms, lasted several hours. When considering different thallus zones, there were big differences in Chl fluorescence parameters. Before HL treatment, Fv/Fm and FII, reached 0.585 and 0.446 in umbilicus zone. Close-to-umbilicus zones showed higher values of 0.649 and 0.523, resp. Marginal zones showed Fv/Fm and FII ranges of 0.599-0.699 and 0.312-0.615. HL treatment led to differential decrease of Fv/Fm and FII in the above-specified zones showing their different sensitivity to HL effects on photosynthesis.
In Czech
Heterogenita fotosyntetických procesů v závislosti na navozené fotoinhibici byla studována pomocí kinetické fluorometrické CCD kamery(FluorCam FL-400) ve stélkách lasallia pustulata. Analýza obrazu umožnila rozlišit zóny stélky vykazující díky anatomickým chrakteristikám odlišnou sensitivitu vůči fotoinhibici. Na úrovni celé stélky byl zaznamenán 23 a 20 % pokles Fv/Fm and FII. Temnotní relaxace vedla k postupnému návratu obou parametrů směrem k původním hodnotám. Byla rozlišena rychlá a pomalá fáze návratu v závislosti na konformačních, respektive resyntetických procesech.
Links
| GA522/03/0754, research and development project |
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| GP206/03/P140, research and development project |
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