ŠMARDA, Jan, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK and Viktor HORVÁTH. Camptothecin induces autophagy of v-myb-transformed monoblasts. Differentiation. Malden, MA 02148, USA: Blackwell Publishing, 2004, vol. 72, No 6, p. 279. ISSN 0301-4681. |
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@article{557993, author = {Šmarda, Jan and Zahradníčková, Eva and Souček, Karel and Horváth, Viktor}, article_location = {Malden, MA 02148, USA}, article_number = {6}, keywords = {Myb; differentiation; programmed cell death; autophagy}, language = {eng}, issn = {0301-4681}, journal = {Differentiation}, title = {Camptothecin induces autophagy of v-myb-transformed monoblasts}, volume = {72}, year = {2004} }
TY - JOUR ID - 557993 AU - Šmarda, Jan - Zahradníčková, Eva - Souček, Karel - Horváth, Viktor PY - 2004 TI - Camptothecin induces autophagy of v-myb-transformed monoblasts JF - Differentiation VL - 72 IS - 6 SP - 279 EP - 279 PB - Blackwell Publishing SN - 03014681 KW - Myb KW - differentiation KW - programmed cell death KW - autophagy N2 - Programmed cell death (PCD) pathways are frequently damaged during processes of malignant transformation. Abrogation of PCD can significantly affect the response of the cancer cell to a therapy. Therefore, understanding of molecular mechanisms regulating PCD in cancer cells is of potential clinical interest. In this study we analyzed PCD pathways in the cells of two leukemia cell lines: human U937 promonocytes and chicken v-myb-transformed BM2 monoblasts. PCD of these cells was induced using three agents: arsenic trioxide induces PCD by elevation of the intracellular level of hydrogen peroxide, cycloheximide acts as inhibitor of proteosynthesis and camptothecin inhibits activity of DNA topoisomerase I thus causing DNA damage. We found the mechanisms of PCD activated in BM2 and U937 cells by these agents partially different. Under conditions when U937 cells undergo caspase-mediated apoptosis, BM2 cells die by caspase-independent pathway. In addition, DNA damage caused by camptothecin results in a switch from apoptosis to autophagy in BM2 cells. Interestingly, camptothecin fails to induce similar effect in U937 cells. v-myb oncogene of avian myeloblastosis virus codes for regulator of proliferation, differentiation and apoptosis. Our results suggest that v-Myb oncoprotein over-expressed in BM2 but not in U937 cells can be significantly engaged in regulation of programmed cell death pathways in leukemic cells. ER -
ŠMARDA, Jan, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK and Viktor HORVÁTH. Camptothecin induces autophagy of v-myb-transformed monoblasts. \textit{Differentiation}. Malden, MA 02148, USA: Blackwell Publishing, 2004, vol.~72, No~6, p.~279. ISSN~0301-4681.
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