Detailed Information on Publication Record
2004
Camptothecin induces autophagy of v-myb-transformed monoblasts
ŠMARDA, Jan, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK and Viktor HORVÁTHBasic information
Original name
Camptothecin induces autophagy of v-myb-transformed monoblasts
Name in Czech
Kamptotecin indukuje autofagii monoblastů transformovaných onkogenem v-myb
Authors
ŠMARDA, Jan (203 Czech Republic, guarantor), Eva ZAHRADNÍČKOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic) and Viktor HORVÁTH (203 Czech Republic)
Edition
Differentiation, Malden, MA 02148, USA, Blackwell Publishing, 2004, 0301-4681
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.481
RIV identification code
RIV/00216224:14310/04:00019786
Organization unit
Faculty of Science
Keywords in English
Myb; differentiation; programmed cell death; autophagy
Změněno: 15/11/2006 13:00, prof. RNDr. Jan Šmarda, CSc.
V originále
Programmed cell death (PCD) pathways are frequently damaged during processes of malignant transformation. Abrogation of PCD can significantly affect the response of the cancer cell to a therapy. Therefore, understanding of molecular mechanisms regulating PCD in cancer cells is of potential clinical interest. In this study we analyzed PCD pathways in the cells of two leukemia cell lines: human U937 promonocytes and chicken v-myb-transformed BM2 monoblasts. PCD of these cells was induced using three agents: arsenic trioxide induces PCD by elevation of the intracellular level of hydrogen peroxide, cycloheximide acts as inhibitor of proteosynthesis and camptothecin inhibits activity of DNA topoisomerase I thus causing DNA damage. We found the mechanisms of PCD activated in BM2 and U937 cells by these agents partially different. Under conditions when U937 cells undergo caspase-mediated apoptosis, BM2 cells die by caspase-independent pathway. In addition, DNA damage caused by camptothecin results in a switch from apoptosis to autophagy in BM2 cells. Interestingly, camptothecin fails to induce similar effect in U937 cells. v-myb oncogene of avian myeloblastosis virus codes for regulator of proliferation, differentiation and apoptosis. Our results suggest that v-Myb oncoprotein over-expressed in BM2 but not in U937 cells can be significantly engaged in regulation of programmed cell death pathways in leukemic cells.
In Czech
V této práci jsme studovali dráhy programované buněčné smrti (PCD) v linii lidských promocytů (U937) a kuřecích monoblastů transformovaných v-myb (BM2). PCD těchto buněk byla idnukována třemi činidly: oxidem arsenitým, cykloheximidem a kamptotecinem. Zjistili jsme, že za podmínek, kdy buňky U937 odumírají apoptózou, buňky BM2 používají jinou dráhu PCD, která je nezávislá na kaspázách. Poškození DNA způsobené u buněk BM2 kamptotecinem, způsobuje autofagii. Naše výsledky dokazují onkoprotein v-Myb může být významně zapojen do procesů regulace PCD v leikemických bnkách.
Links
| GA301/03/1055, research and development project |
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| MSM 143100008, plan (intention) |
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