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@inproceedings{558524,
author = {Hradilová, Jana and Brzobohatý, Břetislav},
address = {Praha},
booktitle = {IV. Mezioborové setkání mladých biologů, biochemiků a chemiků, Chemické listy},
keywords = {AHP5; alternative splicing; two-component system; Arabidopsis thaliana},
language = {eng},
location = {Praha},
isbn = {0009-2770},
pages = {282-282},
publisher = {Česká společnost chemická},
title = {Tissue specific alternative splicing in the gene for AHP5, a histidine-containing phosphotransfer protein from Arabidopsis thaliana},
year = {2004}
}
TY - JOUR
ID - 558524
AU - Hradilová, Jana - Brzobohatý, Břetislav
PY - 2004
TI - Tissue specific alternative splicing in the gene for AHP5, a histidine-containing phosphotransfer protein from Arabidopsis thaliana
PB - Česká společnost chemická
CY - Praha
SN - 00092770
KW - AHP5
KW - alternative splicing
KW - two-component system
KW - Arabidopsis thaliana
N2 - A multistep two-component system is involved in signal transduction in Arabidopsis and consists of a sensory histidine kinase, a phosphotransfer factor and a response regulator. Factors with histidine-containing phosphotransfer domains (AHPs) transfer the phosphoryl group from membrane localised receptor (histidine kinases) to effectors (response regulators) localised in the nucleus. Five AHPs have been identified in Arabidopsis. AHPs are small proteins (14,5 18 kDa), with a predicted acidic isoeletric point (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS. AHP5 comprises six exons and five introns. RNA was prepared using Trizol reagent from different plant tissues: leaves, roots, shoot, flowers, siliques and seedlings. Using RT-PCR we detected two products (AHP5s and AHP5l). Following sequencing, the second intron was found intact at position 211 bp in the longer product (AHP5l). Real time RT-PCR showed that the ratio between spliced and nonspliced products is in tested tissues significant different. Other AHPs (AHP1, AHP2, AHP3 and AHP4) were established to be normally spliced. AHP5l comprises two open reading frames, and in the longer of them the phosphotransfer domain is preserved intact as the original reading frame is not altered. This protein has a predicted molecular mass of 11.7kDa and the putative isoeletric point is 7.72.
ER -
HRADILOVÁ, Jana and Břetislav BRZOBOHATÝ. Tissue specific alternative splicing in the gene for AHP5, a histidine-containing phosphotransfer protein from Arabidopsis thaliana. In \textit{IV. Mezioborové setkání mladých biologů, biochemiků a chemiků, Chemické listy}. Praha: Česká společnost chemická, 2004, p.~282. ISBN~0009-2770.
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