Characterization of the expression pattren of the Arabidopsis
AHP factores gene family

B 2004

Characterization of the expression pattren of the Arabidopsis AHP factores gene family

HRADILOVÁ, Jana and Břetislav BRZOBOHATÝ

Basic information

Original name

Characterization of the expression pattren of the Arabidopsis AHP factores gene family

Authors

HRADILOVÁ, Jana (203 Czech Republic) and Břetislav BRZOBOHATÝ (203 Czech Republic, guarantor)

Edition

Bratislava, 1 pp. Book of abstract, 2004

Publisher

Dni rastlinnej fyziológie X.

Other information

Language

English

Type of outcome

Odborná kniha

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

cytokinin; AHP; gene expression; real time PCR

Abstract

V originále

In this study we focused on expression pattern of a family of AHPs genes in different plant tissues. We also examined the influence of cytokinins on AHP gene expression. AHPs (Arabidopsis Histidine-phosphotransfer factors) are phosphotransfer shuttles in the multistep two-component system of signal transduction. AHPs transfer the phosphoryl group between membrane localised receptors (histidine kinases) and response regulators (transcription factors) localized in the nucleus. This system is thought to be involved in stress, cytokinin and ethylene signalling in Arabidopsis. Cytokinins have been implicated in developmental and growth processes in plants including cell division, shoot meristem initiation, senescence and germination. Five AHPs have been identified until now in Arabidopsis. AHPs are small proteins (14.5 18 kDa), with a predicted acidic isoeletric point (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS with the histidine residue binding the phosphoryl group. Using real time RT-PCR we characterized the expression pattern of AHP genes in adult wild type Arabidopsis thaliana (ecotype Columbia 0). Different plant organs were tested leaves, stems, roots, flowers and siliques. The influence of cytokinins on the level of AHP expression was tested on 8 day-old seedlings treated with the cytokinin benzyladenine (BA) and also on 8 day-old seedlings overproducing endogenous cytokinins. We show that the highest mRNA level of the AHP1 is in roots and highest level of AHP2 is in flowers, on the other hand there were no significant differences among tissues in expression patterns of AHP3 and AHP5. AHP4 was expressed at very low levels in all tissues tested but the highest level was found in leaves. Also most of tested AHPs have low relative expression in seedlings and siliques. A response to cytokinin was detected for AHP1 and AHP4, their steady-state mRNA levels going up. The expression of AHP3 was affected more by exogenous cytokinins and the AHP2 was more responsible to higher level of the endogenous cytokinins. Expression level AHP5 remains unaffected using cytokinins.

Links

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

HRADILOVÁ, Jana and Břetislav BRZOBOHATÝ. Characterization of the expression pattren of the Arabidopsis AHP factores gene family. Bratislava: Dni rastlinnej fyziológie X., 2004, 1 pp. Book of abstract.

@book{558527,
   author = {Hradilová, Jana and Brzobohatý, Břetislav},
   address = {Bratislava},
   keywords = {cytokinin; AHP; gene expression; real time PCR},
   language = {eng},
   location = {Bratislava},
   publisher = {Dni rastlinnej fyziológie X.},
   title = {Characterization of the expression pattren of the Arabidopsis AHP factores gene family},
   year = {2004}
}

TY  - BOOK
ID  - 558527
AU  - Hradilová, Jana - Brzobohatý, Břetislav
PY  - 2004
TI  - Characterization of the expression pattren of the Arabidopsis AHP factores gene family
VL  - Book of abstract
PB  - Dni rastlinnej fyziológie X.
CY  - Bratislava
KW  - cytokinin
KW  - AHP
KW  - gene expression
KW  - real time PCR
N2  - In this study we focused on expression pattern of a family of AHPs genes in different plant tissues. We also examined the influence of cytokinins on AHP gene expression. AHPs (Arabidopsis Histidine-phosphotransfer factors) are phosphotransfer shuttles in the multistep two-component system of signal transduction. AHPs transfer the phosphoryl group between membrane localised receptors (histidine kinases) and response regulators (transcription factors) localized in the nucleus. This system is thought to be involved in stress, cytokinin and ethylene signalling in Arabidopsis. Cytokinins have been implicated in developmental and growth processes in plants including cell division, shoot meristem initiation, senescence and germination. Five AHPs have been identified until now in Arabidopsis. AHPs are small proteins (14.5 18 kDa), with a predicted acidic isoeletric point (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS with the histidine residue binding the phosphoryl group. Using real time RT-PCR we characterized the expression pattern of AHP genes in adult wild type Arabidopsis thaliana (ecotype Columbia 0). Different plant organs were tested leaves, stems, roots, flowers and siliques. The influence of cytokinins on the level of AHP expression was tested on 8 day-old seedlings treated with the cytokinin benzyladenine (BA) and also on 8 day-old seedlings overproducing endogenous cytokinins. We show that the highest mRNA level of the AHP1 is in roots and highest level of AHP2 is in flowers, on the other hand there were no significant differences among tissues in expression patterns of AHP3 and AHP5. AHP4 was expressed at very low levels in all tissues tested but the highest level was found in leaves. Also most of tested AHPs have low relative expression in seedlings and siliques. A response to cytokinin was detected for AHP1 and AHP4, their steady-state mRNA levels going up. The expression of AHP3 was affected more by exogenous cytokinins and the AHP2 was more responsible to higher level of the endogenous cytokinins. Expression level AHP5 remains unaffected using cytokinins.
ER  -

HRADILOVÁ, Jana and Břetislav BRZOBOHATÝ. \textit{Characterization of the expression pattren of the Arabidopsis AHP factores gene family}. Bratislava: Dni rastlinnej fyziológie X., 2004, 1 pp. Book of abstract.

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