Immunofluorescence staining of the insulin-like growth factor-I in the ventral and dorsal spinal roots

SVÍŽENSKÁ, Ivana, Petr DUBOVÝ and Ilona KLUSÁKOVÁ. Immunofluorescence staining of the insulin-like growth factor-I in the ventral and dorsal spinal roots. Scripta medica. Brno: Faculty of Medicine, Masaryk University, 2002, vol. 2002, No 75, p. 336-337, 1 pp. ISSN 1211-3395.

Basic information

• Original name: Immunofluorescence staining of the insulin-like growth factor-I in the ventral and dorsal spinal roots
• Name in Czech: Imunofluorescenční zbarvení IGF-I ve ventrálních a dorzálních míšních kořenech
• Authors: SVÍŽENSKÁ, Ivana (203 Czech Republic), Petr DUBOVÝ (203 Czech Republic, guarantor) and Ilona KLUSÁKOVÁ (203 Czech Republic).
• Edition: Scripta medica, Brno, Faculty of Medicine, Masaryk University, 2002, 1211-3395.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/02:00010398
• Organization unit: Faculty of Medicine
• Keywords in English: Schwann cells; IGF-I; regeneration; peripheral nerve; motor; sensory axons; Lucia G
• Tags: IGF-I, Lucia G, motor, Peripheral nerve, Regeneration, Schwann cells, sensory axons

Abstract

To reveal whether there are differences in appearance of IGF-I in microenvironment of efferent and afferent axons authors have analysed immunohistochemically the expression of the IGF-I molecules in the intact ventral and dorsal roots of the spinal cord in rat. The results of immunostaining were viewed and digitised in a Leica-DMBL fluorescence microscope equipped with a DC-100 camera. A brightness of fluorescence immunolabeling was measured by means of an image analyzing system (Lucia G). The results revealed a higher IGF-I immunoreactivity in the dorsal roots than in the ventral ones.

Abstract (in Czech)

Autoři analyzovali imunohistochemicky expresi molekul IGF-I v intaktních ventrálních a dorzálních míšních kořenech u laboratorního potkana. Výsledky imunohistochemické reakce byly vizualizovány a digitalizovány ve fluorescenčním mikroskopu Leica vybaveném kamerou DC-100. Intenzita fluorescenčního zbarvení byla měřena pomocí analýzy obrazu (Lucia G). Výsledky ukázaly vyšší výskyt molekul IGF-I v dorzálních míšních kořenech.

Links

• GA309/00/0407, research and development project: Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves