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@article{560044,
author = {Stoneberg Holt, Sierra Dawn and Holt, Jason Andrew},
article_number = {173},
keywords = {deletion; DNA target regions; indel; insertion; Poaceae; software; trnL intron},
language = {eng},
issn = {1471-2105},
journal = {BMC Bioinformatics},
title = {The InDeVal insertion/deletion evaluation tool: a program for finding target regions in DNA sequences and for aiding in sequence comparison},
url = {http://www.biomedcentral.com/content/pdf/1471-2105-5-173.pdf},
volume = {5},
year = {2004}
}
TY - JOUR
ID - 560044
AU - Stoneberg Holt, Sierra Dawn - Holt, Jason Andrew
PY - 2004
TI - The InDeVal insertion/deletion evaluation tool: a program for finding target regions in DNA sequences and for aiding in sequence comparison
JF - BMC Bioinformatics
VL - 5
IS - 173
SP - 1-24
EP - 1-24
PB - BioMed Central
SN - 14712105
KW - deletion
KW - DNA target regions
KW - indel
KW - insertion
KW - Poaceae
KW - software
KW - trnL intron
UR - http://www.biomedcentral.com/content/pdf/1471-2105-5-173.pdf
N2 - Background The program InDeVal was originally developed to help researchers find known regions of insertion/deletion activity (with the exception of isolated single-base indels) in newly determined Poaceae trnL-F sequences and compare them with 533 previously determined sequences. It is supplied with input files designed for this purpose. More broadly, the program is applicable for finding specific target regions (referred to as "variable regions") in DNA sequence. A variable region is any specific sequence fragment of interest, such as an indel region, a codon or codons, or sequence coding for a particular RNA secondary structure. Implementation InDeVal input is DNA sequence and a template file (sequence flanking each variable region). Additional files contain the variable regions and user-defined messages about the sequence found within them (e.g., taxa sharing each of the different indel patterns). Variable regions are found by determining the position of flanking sequence (referred to as "conserved regions") using the LPAM (Length-Preserving Alignment Method) algorithm. This algorithm was designed for InDeVal and is described here for the first time. InDeVal output is an interactive display of the analyzed sequence, broken into user-defined units. Once the user is satisfied with the organization of the display, the information can be exported to an annotated text file. Conclusions InDeVal can find multiple variable regions simultaneously (28 indel regions in the Poaceae trnL-F files) and display user-selected messages specific to the sequence variants found. InDeVal output is designed to facilitate comparison between the analyzed sequence and previously evaluated sequence. The program's sensitivity to different levels of nucleotide and/or length variation in conserved regions can be adjusted. InDeVal is currently available for Windows in Additional file 1 or from http://www.sci.muni.cz/botany/elzdroje/indeval/.
ER -
STONEBERG HOLT, Sierra Dawn and Jason Andrew HOLT. The InDeVal insertion/deletion evaluation tool: a program for finding target regions in DNA sequences and for aiding in sequence comparison. \textit{BMC Bioinformatics}. BioMed Central, 2004, vol.~5, No~173, p.~1-24. ISSN~1471-2105.
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