Indel patterns of the plastid DNA trnL-trnF region within the
genus Poa (Poaceae)

J 2004

Indel patterns of the plastid DNA trnL-trnF region within the genus Poa (Poaceae)

STONEBERG HOLT, Sierra Dawn, Lucie HOROVÁ and Petr BUREŠ

Basic information

Original name

Indel patterns of the plastid DNA trnL-trnF region within the genus Poa (Poaceae)

Name in Czech

Indelové paterny trnL-trnF regionu plastidové DNA v rámci rodu Poa (Poaceae)

Authors

STONEBERG HOLT, Sierra Dawn (840 United States of America, guarantor), Lucie HOROVÁ (203 Czech Republic) and Petr BUREŠ (203 Czech Republic)

Edition

Journal of Plant Research, Tokyo, Springer-Verlag Tokyo, 2004, 0918-9440

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Japan

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.224

RIV identification code

RIV/00216224:14310/04:00010417

Organization unit

Faculty of Science

UT WoS

000224761200008

Keywords in English

Indel patterns; infrageneric variability; Poa; Poaceae; trnL intron; trnL-trnF IGS

Abstract

ORIG CZ

V originále

The use of insertion/deletion (indel) patterns from sequences of the trnL intron and trnL-F intergenic spacer (IGS) in finding plastid genome types of the genus Poa L. was studied. New sequences for 23 taxa (P. alpina, P. badensis, P. bulbosa, P. crassipes, P. molinerii, P. annua, P. chaixii, P. granitica, P. pratensis, P. sibirica, P. remota, P. botryoides, P. cenisia, P. compressa, P. laxa, P. margilicola, P. media, P. nemoralis, P. palustris, P. pannonica, P. pirinica, P. riphaea, and P. sejuncta) and 18 previously published sequences, which represent 11 of the 13 sections listed for Poa in Flora Europaea, were investigated. Indel patterns, despite sampling less than 0.7 % of the plastid genome, produced four taxa groupings that were congruent with the major divisions obtained in intensive, previously published restriction site studies. Insertion/deletion events in the trnL intron and trnL-trnF IGS were in nearly all cases unique to a single pattern group and thus provided almost no information about relationships among these groups. Indels did, however, provide a meaningful infrageneric classification criterion for Poa. They can serve as useful tools in studying relationships within this genus.

In Czech

Pro klasifikaci skupin taxonů z rodu Poa s různými typy plastidových genomů byly použity indely (inzerce/delece) z trnL intronu a trnL-trnF mezigenového spaceru (IGS). V rámci 11 z 13 sekcí rodu Poa prezentovaných ve díle Flora Europaea bylo nově sekvencováno celkem 23 taxonů (P. alpina, P. badensis, P. bulbosa, P. crassipes, P. molinerii, P. annua, P. chaixii, P. granitica, P. pratensis, P. sibirica, P. remota, P. botryoides, P. cenisia, P. compressa, P. laxa, P. margilicola, P. media, P. nemoralis, P. palustris, P. pannonica, P. pirinica, P. riphaea, and P. sejuncta) a dále 18 sekvencí, dostupných z databáze NCBI. Sekvenční paterny indelů, zahrnující méně než 0.7 % plastidového genomu, potvrdily čtyři skupiny taxonů, již dříve vymezené na základě restrikční analýzy celé plastidové DNA. Zjištěné indely byly téměř vždy unikátní pro danou skupinu a nebylo je proto možné využít k posouzení vzájemné příbuznosti skupin. Byly však užitečné pro infragenerickou klasifikaci rodu Poa.

Links

MSM 143100010, plan (intention)

Name: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministry of Education, Youth and Sports of the CR, Spatiotemporal biodiversity dynamics in ecosystems of Central Europe

Detailed Information on Publication Record