Indel patterns of the plastid DNA trnL-trnF region within the genus Poa (Poaceae)

STONEBERG HOLT, Sierra Dawn, Lucie HOROVÁ and Petr BUREŠ. Indel patterns of the plastid DNA trnL-trnF region within the genus Poa (Poaceae). Journal of Plant Research. Tokyo: Springer-Verlag Tokyo, 2004, vol. 117, No 5, p. 393-407. ISSN 0918-9440.

Basic information

• Original name: Indel patterns of the plastid DNA trnL-trnF region within the genus Poa (Poaceae)
• Name in Czech: Indelové paterny trnL-trnF regionu plastidové DNA v rámci rodu Poa (Poaceae)
• Authors: STONEBERG HOLT, Sierra Dawn (840 United States of America, guarantor), Lucie HOROVÁ (203 Czech Republic) and Petr BUREŠ (203 Czech Republic).
• Edition: Journal of Plant Research, Tokyo, Springer-Verlag Tokyo, 2004, 0918-9440.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Japan
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.224
• RIV identification code: RIV/00216224:14310/04:00010417
• Organization unit: Faculty of Science
• UT WoS: 000224761200008
• Keywords in English: Indel patterns; infrageneric variability; Poa; Poaceae; trnL intron; trnL-trnF IGS
• Tags: Indel patterns, infrageneric variability, Poa, Poaceae, trnL intron, trnL-trnF IGS
• Tags: International impact, Reviewed

Abstract

The use of insertion/deletion (indel) patterns from sequences of the trnL intron and trnL-F intergenic spacer (IGS) in finding plastid genome types of the genus Poa L. was studied. New sequences for 23 taxa (P. alpina, P. badensis, P. bulbosa, P. crassipes, P. molinerii, P. annua, P. chaixii, P. granitica, P. pratensis, P. sibirica, P. remota, P. botryoides, P. cenisia, P. compressa, P. laxa, P. margilicola, P. media, P. nemoralis, P. palustris, P. pannonica, P. pirinica, P. riphaea, and P. sejuncta) and 18 previously published sequences, which represent 11 of the 13 sections listed for Poa in Flora Europaea, were investigated. Indel patterns, despite sampling less than 0.7 % of the plastid genome, produced four taxa groupings that were congruent with the major divisions obtained in intensive, previously published restriction site studies. Insertion/deletion events in the trnL intron and trnL-trnF IGS were in nearly all cases unique to a single pattern group and thus provided almost no information about relationships among these groups. Indels did, however, provide a meaningful infrageneric classification criterion for Poa. They can serve as useful tools in studying relationships within this genus.

Abstract (in Czech)

Pro klasifikaci skupin taxonů z rodu Poa s různými typy plastidových genomů byly použity indely (inzerce/delece) z trnL intronu a trnL-trnF mezigenového spaceru (IGS). V rámci 11 z 13 sekcí rodu Poa prezentovaných ve díle Flora Europaea bylo nově sekvencováno celkem 23 taxonů (P. alpina, P. badensis, P. bulbosa, P. crassipes, P. molinerii, P. annua, P. chaixii, P. granitica, P. pratensis, P. sibirica, P. remota, P. botryoides, P. cenisia, P. compressa, P. laxa, P. margilicola, P. media, P. nemoralis, P. palustris, P. pannonica, P. pirinica, P. riphaea, and P. sejuncta) a dále 18 sekvencí, dostupných z databáze NCBI. Sekvenční paterny indelů, zahrnující méně než 0.7 % plastidového genomu, potvrdily čtyři skupiny taxonů, již dříve vymezené na základě restrikční analýzy celé plastidové DNA. Zjištěné indely byly téměř vždy unikátní pro danou skupinu a nebylo je proto možné využít k posouzení vzájemné příbuznosti skupin. Byly však užitečné pro infragenerickou klasifikaci rodu Poa.

Links

• MSM 143100010, plan (intention): Name: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministry of Education, Youth and Sports of the CR, Spatiotemporal biodiversity dynamics in ecosystems of Central Europe