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@article{560975,
author = {Mornstein, Vojtěch and Bečvářová, Pavlína and Nový, Jindřich and Škorpíková, Jiřina and Janisch, Roman},
article_location = {Zagreb},
article_number = {Supp. 2},
keywords = {Ultrasound; cytoskeleton; immunofluorescence; fast Fourier transform; microtubules; image analysis},
language = {eng},
issn = {1330-4917},
journal = {Liječnički Vjesnik},
note = {Abstract, XVI. European Congress of Ultrasound in Medicine and Biology, Zagreb, Croatia, 5.-8. June 2004},
title = {How to evaluate the cytoskeletal response to the action of ultrasound?},
volume = {126},
year = {2004}
}
TY - JOUR
ID - 560975
AU - Mornstein, Vojtěch - Bečvářová, Pavlína - Nový, Jindřich - Škorpíková, Jiřina - Janisch, Roman
PY - 2004
TI - How to evaluate the cytoskeletal response to the action of ultrasound?
JF - Liječnički Vjesnik
VL - 126
IS - Supp. 2
SP - 168-168
EP - 168-168
PB - Hrvatski Liječnički Zbor
SN - 13304917
N1 - Abstract, XVI. European Congress of Ultrasound in Medicine and Biology, Zagreb, Croatia, 5.-8. June 2004
KW - Ultrasound
KW - cytoskeleton
KW - immunofluorescence
KW - fast Fourier transform
KW - microtubules
KW - image analysis
N2 - Cytoskeleton changes are usually qualitatively described in vague terms like rarefaction, aggregation etc. An objective method for quantifying cytoskeletal changes was not yet published. We have used HeLa cells cultured in a standard way. Cells were exposed to 1 MHz ultrasound (1 and 2 W.cm-2) for 10 min. The microtubules were detected by a fluorescence microscope by immunofluorescence method. Method of cell structure analysis is based on frequency domain filtering using the discrete Fourier transform. We designed filters enabling extraction of significant information about structural changes of microtubules from micrographs. For comparison purposes we applied further analysis which assesses the degree of damage of microtubules and assigns it a numeric descriptor computed as the ratio of area occupied by microtubules to the total area of the cell. Our mathematical approach was sufficiently sensitive for assessment of microtubular network changes.
ER -
MORNSTEIN, Vojtěch, Pavlína BEČVÁŘOVÁ, Jindřich NOVÝ, Jiřina ŠKORPÍKOVÁ and Roman JANISCH. How to evaluate the cytoskeletal response to the action of ultrasound? \textit{Liječnički Vjesnik}. Zagreb: Hrvatski Liječnički Zbor, 2004, vol.~126, Supp. 2, p.~168-168. ISSN~1330-4917.
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