HAVLIŠ, Jan and Andrej SHEVCHENKO. Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2004, vol. 76, No 11, p. 3029-3036, 7 pp. ISSN 0003-2700.
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Basic information
Original name Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry
Name (in English) Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry
Authors HAVLIŠ, Jan and Andrej SHEVCHENKO.
Edition Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2004, 0003-2700.
Other information
Type of outcome Article in a journal
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.450
Organization unit Faculty of Science
UT WoS 000221755200015
Keywords in English COMPARATIVE PROTEOMICS; PEPTIDE; MIXTURES; O-18; PHOSPHORYLATION; PURIFICATION; QUANTITATION; EXPRESSION; DIGESTION; STANDARDS
Tags COMPARATIVE PROTEOMICS, DIGESTION, Expression, MIXTURES, O-18, peptide, phosphorylation, purification, quantitation, standards
Tags International impact, Reviewed
Changed by Changed by: doc. Mgr. Jan Havliš, Dr., učo 743. Changed: 2/7/2009 18:59.
Abstract (in English)
A combination of nanoelectrospray tandem mass spectrometry and O-18-labeled peptide internal standards was applied for the absolute quantification of proteins from their in-solution and in-gel tryptic digests. Although absolute quantification from in-solution digests was accurate, we observed that in-gel digestion compromised the quantification accuracy by affecting the recovery of individual peptides and, therefore, the provided estimates might be strongly influenced by the selection of reference peptides. Under optimized experimental conditions, it was possible to provide a semiquantitative estimate of the absolute amount of gel separated proteins within better than 50% error margin.
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