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@article{561459,
author = {Kuňák, Michal and Kučera, Igor and Van Spanning, Rob J.M.},
article_location = {New York},
article_number = {2},
keywords = {denitrification; nitrite reductase; nitric oxide reductase; substrate inhibition; concentration oscillations},
language = {eng},
issn = {0003-9861},
journal = {Archives of biochemistry and biophysics},
title = {Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells},
url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4D0Y364-2&_coverDate=09%2F15%2F2004&_alid=223287397&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6701&_sort=d&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=e269cd2afbc12bda4bc9},
volume = {429},
year = {2004}
}
TY - JOUR
ID - 561459
AU - Kuňák, Michal - Kučera, Igor - Van Spanning, Rob J.M.
PY - 2004
TI - Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells
JF - Archives of biochemistry and biophysics
VL - 429
IS - 2
SP - 237-243
EP - 237-243
PB - Academic Press
SN - 00039861
KW - denitrification
KW - nitrite reductase
KW - nitric oxide reductase
KW - substrate inhibition
KW - concentration oscillations
UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4D0Y364-2&_coverDate=09%2F15%2F2004&_alid=223287397&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6701&_sort=d&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=e269cd2afbc12bda4bc9
N2 - Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory chain.
ER -
KUŇÁK, Michal, Igor KUČERA and Rob J.M. VAN SPANNING. Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells. \textit{Archives of biochemistry and biophysics}. New York: Academic Press, 2004, vol.~429, No~2, p.~237-243. ISSN~0003-9861.
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