Nitric oxide oscillations in Paracoccus denitrificans: the
effects of environmental factors and of segregating nitrite
reductase and nitric oxide reductase into separate cells

J 2004

Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells

KUŇÁK, Michal, Igor KUČERA and Rob J.M. VAN SPANNING

Basic information

Original name

Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells

Name in Czech

Oscilace oxidu dusnatého u Paracoccus denitrificans: vliv faktorů prostředí o oddělení nitritreduktasy a reduktasy oxidu dusnatého do různých buněk

Authors

KUŇÁK, Michal (203 Czech Republic), Igor KUČERA (203 Czech Republic, guarantor) and Rob J.M. VAN SPANNING (528 Netherlands)

Edition

Archives of biochemistry and biophysics, New York, Academic Press, 2004, 0003-9861

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.657

RIV identification code

RIV/00216224:14310/04:00010556

Organization unit

Faculty of Science

UT WoS

000223563600015

Keywords in English

denitrification; nitrite reductase; nitric oxide reductase; substrate inhibition; concentration oscillations

Abstract

ORIG CZ

V originále

Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory chain.

In Czech

Oxid dusnatý je meziprodukt denitrifikace, který vzniká z dusitanu a je dále přeměňován přes oxid dusný na dusík. Byl sledován vliv nízkých koncentrací protonoforu karbonylkyanid-m-chlorfenylhydrazonu na časový průběh koncentrací rozpuštěných plynů. Zatímco u NO byly zjištěny oscilace, N2O se postupně hromadil. Frekvence a tvar oscilací NO vyvolaných protonoforem byly ovlivňovány teplotou a koncentrací elektronového donoru TMPD v souladu s pozorovaným působením na aktivity jednotlivých enzymů. Ukázali jsme existenci intervalu pH ve kterém NO osciluje dokonce bez přidání rozpojovače. Výskyt oscilací NO ve směsi mutant ukazuje, že nemohou být způsobeny kompeticí obou enzymů o redukční ekvivalenty z jednoho společného respiračního řetězce.

Links

MSM 143100005, plan (intention)

Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism

Citovat

KUŇÁK, Michal, Igor KUČERA and Rob J.M. VAN SPANNING. Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells. Archives of biochemistry and biophysics. New York: Academic Press, 2004, vol. 429, No 2, p. 237-243. ISSN 0003-9861.

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   author = {Kuňák, Michal and Kučera, Igor and Van Spanning, Rob J.M.},
   article_location = {New York},
   article_number = {2},
   keywords = {denitrification; nitrite reductase; nitric oxide reductase; substrate inhibition; concentration oscillations},
   language = {eng},
   issn = {0003-9861},
   journal = {Archives of biochemistry and biophysics},
   title = {Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4D0Y364-2&_coverDate=09%2F15%2F2004&_alid=223287397&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6701&_sort=d&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=e269cd2afbc12bda4bc9},
   volume = {429},
   year = {2004}
}

TY  - JOUR
ID  - 561459
AU  - Kuňák, Michal - Kučera, Igor - Van Spanning, Rob J.M.
PY  - 2004
TI  - Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells
JF  - Archives of biochemistry and biophysics
VL  - 429
IS  - 2
SP  - 237-243
EP  - 237-243
PB  - Academic Press
SN  - 00039861
KW  - denitrification
KW  - nitrite reductase
KW  - nitric oxide reductase
KW  - substrate inhibition
KW  - concentration oscillations
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4D0Y364-2&_coverDate=09%2F15%2F2004&_alid=223287397&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6701&_sort=d&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=e269cd2afbc12bda4bc9
N2  - Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory chain.
ER  -

KUŇÁK, Michal, Igor KUČERA and Rob J.M. VAN SPANNING. Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells. \textit{Archives of biochemistry and biophysics}. New York: Academic Press, 2004, vol.~429, No~2, p.~237-243. ISSN~0003-9861.

Detailed Information on Publication Record