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@article{561550,
author = {Stoneberg Holt, Sierra Dawn and Horová, Lucie and Bureš, Petr and Janeček, Josef and Černoch, Vladimír},
article_location = {Praha},
article_number = {2},
keywords = {cultivar characterization; Harmonie; Moravanka; non-coding plastid DNA sequence; Slezanka; trnL intron; trnL-trnF intergenic spacer; turf grass},
language = {eng},
issn = {1214-1178},
journal = {Plant, Soil and Environment},
title = {The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties},
volume = {51},
year = {2005}
}
TY - JOUR
ID - 561550
AU - Stoneberg Holt, Sierra Dawn - Horová, Lucie - Bureš, Petr - Janeček, Josef - Černoch, Vladimír
PY - 2005
TI - The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties
JF - Plant, Soil and Environment
VL - 51
IS - 2
SP - 94-99
EP - 94-99
PB - Czech Academy of Agricultural Sciences
SN - 12141178
KW - cultivar characterization
KW - Harmonie
KW - Moravanka
KW - non-coding plastid DNA sequence
KW - Slezanka
KW - trnL intron
KW - trnL-trnF intergenic spacer
KW - turf grass
N2 - The characterization of crop cultivars (varieties) will come to depend increasingly on molecular characters in addition to traditional morphological and agronomic characters. Three cultivars of Kentucky bluegrass (Poa pratensis L.), developed by the Plant Breeding Station Hladké Životice (PBHŽ), were characterized using sequences and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) patterns from the non-coding plastid trnL-F region (trnL intron, 549 bp, and trnL-trnF intergenic spacer [IGS], 344 to 364 bp). These characters could be readily and repeatably determined not only for mature plants, but also for seedlings (less than 12 weeks old), which are difficult to distinguish morphologically. The method is quick and sensitive. When restricted with a combination of BsaJ I and Bsm I, cultivar Slezanka has one major band, Moravanka has two, and Harmonie has three. When restricted with Alu I, the heaviest band migrates most slowly for Slezanka. It is expected that many Kentucky bluegrass cultivars will share the same trnL-F sequence, so these characters alone are not sufficient for variety identification.
ER -
STONEBERG HOLT, Sierra Dawn, Lucie HOROVÁ, Petr BUREŠ, Josef JANEČEK and Vladimír ČERNOCH. The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties. \textit{Plant, Soil and Environment}. Praha: Czech Academy of Agricultural Sciences, 2005, vol.~51, No~2, p.~94-99. ISSN~1214-1178.
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