Detailed Information on Publication Record
2004
Changes of immunofluorescence staining for ECM molecules in the rat dorsal root ganglia following chronic compression of the peripheral nerve
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Radim JANČÁLEKBasic information
Original name
Changes of immunofluorescence staining for ECM molecules in the rat dorsal root ganglia following chronic compression of the peripheral nerve
Name in Czech
Změny imunofluorescenčního barvení molekul ECM ve spinálních gangliích potkana po chronické kompresi periferního nervu.
Authors
DUBOVÝ, Petr (203 Czech Republic, guarantor), Ilona KLUSÁKOVÁ (203 Czech Republic) and Radim JANČÁLEK (203 Czech Republic)
Edition
Journal of Molecular Histology, Kluwer Academic Publishers, 2004, 1567-2379
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/04:00010570
Organization unit
Faculty of Medicine
Keywords in English
extracellular matrix dorsal root ganglion nerve compression
Změněno: 26/6/2009 10:48, prof. RNDr. Petr Dubový, CSc.
V originále
The units of neuronal body-satellite cells in the dorsal root ganglia (DRG) are surrounded by the basal lamina and extracellular matrix (ECM) produced by the satellite cells. The neurons shift from normal maintenance and neurotransmission toward survival and regeneration after axonal injury. However, scarce information is available on reaction of satellite cells following axotomy. The present experiments demonstrate changes of immunofluorescence staining for laminin-1, chondroitin sulfate proteoglycan (CSP), tenascin (TEN), thrombospondin (THS) and fibronectin (FN) in the DRG after intercostal nerve ligation. Twenty adult rats were deeply anesthetized and left intercostal nerves (8-12) were ligated by 6-0 Mersilk thread. Animals were allowed to survive for 1 or 2 weeks. The corresponding DRG (Th8-12) of both sides were removed and immersed in Zamboni fixation. Intact DRG (Th8-12) were removed and fixed from other 4 rats. Longitudinal cryostat sections (12 ľm) through ipsilateral, contralateral and intact DRG were incubated for indirect immunofluorescence staining to detect laminin-1, CSP, TEN, THS and FN. An intensity of immunofluorescence staining was compared between digitalized pictures of ipsilateral and contralateral DRG by image analysis system (Lucia G). Ligature of the intercostal nerve induced enlargement of satellite cell sheaths in the ipsilateral DRG. In comparison to the contralateral and intact DRG, a laminin immunofluorescence was reduced around the neuron-satellite cell units in some regions of the sections through ipsilateral DRG. In contrast, immunofluorescence brightness for CSP, TEN, THS and FN was increased in enlarged satellite cell sheaths of ipsilateral comparing contralateral DRG. The quantitative changes of ECM molecules may be involved in external conditions that stimulate a growth of sympathetic sprouts in DRG after compression of peripheral nerve.
In Czech
Funkční a morfologické jednotky neuron-satelitní buňky lokalizované ve spinálních gangliích jsou obklopeny bazální laminou a extracelulární matrix produkovanou satelitními buňkami. Ačkoliv jsou reakce satelitních buněk faktorem, který pravděpodobně ovlivňuje proces regenerace neuronu po poškození neuronu, je informací o těchto reakcích stále nedostatek. Práce se zabývá změnami imunofluorescenčního barvení pro laminin-1, chondroitin sulfát, tenascin, trombospondin a fibronektin ve spinálních gangliích potkana po ligatuře mezižeberních nervů.
Links
| GA309/03/1199, research and development project |
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