Magnetic supports for molecular diagnostics of microorganismus

ŠPANOVÁ, Alena, Bohuslav RITTICH, Milan BENEŠ and Daniel HORÁK. Magnetic supports for molecular diagnostics of microorganismus. In International Symposium on the Separation of Proteins, Peptides and Polynucleotides. Franfurt am Main: Dechema, 2004, p. 64-64.

Basic information

• Original name: Magnetic supports for molecular diagnostics of microorganismus
• Name in Czech: Magnetické nosiče pro molekulární diagnostiku mikroorganismů
• Authors: ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Bohuslav RITTICH (203 Czech Republic), Milan BENEŠ (203 Czech Republic) and Daniel HORÁK (203 Czech Republic).
• Edition: Franfurt am Main, International Symposium on the Separation of Proteins, Peptides and Polynucleotides, p. 64-64, 1 pp. 2004.
• Publisher: Dechema

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/04:00011725
• Organization unit: Faculty of Science
• Keywords in English: Magnetic carriers; solid-phase reversible immobilization; PCR; RAPD
• Tags: magnetic carriers, PCR, RAPD, solid-phase reversible immobilization

Abstract

For solid-phase reversible immobilization (SPRI), two types of magnetic supports were developed. The first ones included magnetic nonporous microparticles of P(HEMA-co-EDMA), the second one cobalt ferrite coated with alginic acid.The quality of isolated DNA was tested by PCR and/or RAPD.

Abstract (in Czech)

Pro vratnou imobilizaci na tuhé fázi byly vyvinuty dva typy nosičů. První zahrnujw magnetické neporézní mikročástice na bázi P(HEMA-co-EDMA), druhý ferity kobaltu pokryté kyselinou alginovou. Kvalita izolované DNA byla testována pomocí PCR a RAPD.

Links

• GA202/02/1361, research and development project: Name: Využití magnetických nosičů s imobilizovanými selektivními ligandy v DNA diagnostice

Investor: Czech Science Foundation, The use of magnetic carriers with immobilized selective ligands in DNA diagnostics

• QD1377, research and development project: Name: Vývoj metod hodnocení a rychlé identifikace vybraných bakterií mléčného kvašení s využitím metod molekulární biologie

Investor: Ministry of Agriculture of the CR, Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods

• 1G46045, research and development project: Name: Molekulárně biologická analýza tuzemského fondu produkčních kmenů baktérií mléčného kvašení.

Investor: Ministry of Agriculture of the CR, Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods