Detailed Information on Publication Record
2004
ASSOCIATION OF FUNCTIONAL SINGLE NUCLEOTIDE POLYMORPHISMS IN MATRIX METALLOPROTEINASES (MMPS) GENES WITH COLORECTAL AND BREAST CANCER PROGRESSION
JURAJDA, Michal and Jiří VÁCHABasic information
Original name
ASSOCIATION OF FUNCTIONAL SINGLE NUCLEOTIDE POLYMORPHISMS IN MATRIX METALLOPROTEINASES (MMPS) GENES WITH COLORECTAL AND BREAST CANCER PROGRESSION
Name in Czech
asociace funkčních SNP v genech matrixmetalloproteináz s progresí karcinomu kolorekta a prsu
Authors
JURAJDA, Michal (203 Czech Republic, guarantor) and Jiří VÁCHA (203 Czech Republic)
Edition
Physiol Res, Praha, Institute of Physiology, Academy of Sci, 2004, 0862-8408
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.140
RIV identification code
RIV/00216224:14110/04:00010701
Organization unit
Faculty of Medicine
Keywords in English
cancer; MMPs; gene polymorphisms
Tags
Změněno: 31/5/2005 11:00, MUDr. Michal Jurajda, Ph.D.
V originále
The degradation of the microenvironment of cancer cells, composed of extracellular matrix (ECM), plays an important role in tumor progression and development of metastases. The most of this degradation is mediated by matrix metalloproteinases. The increased levels of MMPs activity in tumor tissue were reported. Functional promoter single nucleotide polymorphisms (SNP) in MMP-1 and MMP-3 genes increase their transcription levels and consequently their enzymatic activity. An insertion of G at -1607 bp in MMP-1 promoter creates an Ets transcription factor family binding site and thus increases MMP-1 transcription. A deletion of A at -1171 bp increases MMP-3 transcription. These polymorphisms probably play an important role in tumor progression and metastasis. Recently, several studies reported associations of these polymorphisms with breast, colorectal, lung and renal carcinomas. Thus we have genotyped 150 patients with colorectal carcinoma and 164 patients with breast cancer for above mentioned SNPs and analyzed allelic frequencies in subgroups with and without metastases. Our results show that there is a weak association between 5A allele and metastases development in colorectal cancer patients (p=0.04375). Since both MMP genes are located in chromosome 11 we have examined their linkage disequilibrium by chi-square test. 1G allele of MMP-1 promoter is closely linked with 5A allele of MMP-3 promoter (p<0,01). The results suggest the role of studied polymorphisms in metastases development is unequivocal.
In Czech
The degradation of the microenvironment of cancer cells, composed of extracellular matrix (ECM), plays an important role in tumor progression and development of metastases. The most of this degradation is mediated by matrix metalloproteinases. The increased levels of MMPs activity in tumor tissue were reported. Functional promoter single nucleotide polymorphisms (SNP) in MMP-1 and MMP-3 genes increase their transcription levels and consequently their enzymatic activity. An insertion of G at -1607 bp in MMP-1 promoter creates an Ets transcription factor family binding site and thus increases MMP-1 transcription. A deletion of A at -1171 bp increases MMP-3 transcription. These polymorphisms probably play an important role in tumor progression and metastasis. Recently, several studies reported associations of these polymorphisms with breast, colorectal, lung and renal carcinomas. Thus we have genotyped 150 patients with colorectal carcinoma and 164 patients with breast cancer for above mentioned SNPs and analyzed allelic frequencies in subgroups with and without metastases. Our results show that there is a weak association between 5A allele and metastases development in colorectal cancer patients (p=0.04375). Since both MMP genes are located in chromosome 11 we have examined their linkage disequilibrium by chi-square test. 1G allele of MMP-1 promoter is closely linked with 5A allele of MMP-3 promoter (p<0,01). The results suggest the role of studied polymorphisms in metastases development is unequivocal.
Links
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