Experiments for Correlating Quaternary Carbons in RNA Bases

FIALA, Radovan, Markéta MUNZAROVÁ and Vladimír SKLENÁŘ. Experiments for Correlating Quaternary Carbons in RNA Bases. Online. In 19th NMR Valtice. 1st ed. Brno: Masarykova univerzita, 2004. p. 33. ISBN 80-210-3352-5. [citováno 2024-04-23]

Basic information

• Original name: Experiments for Correlating Quaternary Carbons in RNA Bases
• Name in Czech: NMR experimenty pro korelaci kvarterních uhlíků v bazích RNA
• Authors: FIALA, Radovan (203 Czech Republic, guarantor), Markéta MUNZAROVÁ (203 Czech Republic) and Vladimír SKLENÁŘ (203 Czech Republic)
• Edition: 1. vyd. Brno, 19th NMR Valtice, p. 33-33, 2004.
• Publisher: Masarykova univerzita

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/04:00010705
• Organization unit: Faculty of Science
• ISBN: 80-210-3352-5
• UT WoS: 000222502200002
• Keywords in English: NMR; spin-spin coupling; RNA; nucleic acid; quaternary carbon
• Tags: NMR, nucleic acid, quaternary carbon, RNA, spin-spin coupling

Abstract

A set of triple-resonance two-dimensional NMR experiments is presented that can be used for correlating all quaternary carbons in RNA bases to one or more of the base protons. The experiments make use of either three-bond proton-carbon couplings and one selective INEPT step (long-range selective HSQC experiment) to transfer the magnetization between a proton and the carbon of interest and back, or they rely on one- and/or two-bond heteronuclear (the H(CN)C and H(N)C experiments) or carbon-carbon couplings (the H(C)C experiment) and multiple INEPT transfer steps. The effect of the large one-bond carbon-carbon coupling in t1 is removed by a constant time evolution or by a selective refocusing. The sensitivity of all the experiments proposed is high enough to obtain a spectrum of 0.5 mM 25-mer RNA stem-loop in four hours or less on a 600 MHz (1H) spectrometer. The results show that the experiments are also applicable to samples containing agents for weak molecular alignment.

Abstract (in Czech)

Práce představuje nové heteronukleární 2D NMR experimenty pro korelaci všech kvarterních uhlíků v bázích RNA s jedním nebo více jádry vodíku. Experimenty jsou založeny na jedno, dvou nebo třívazebných interakcích a několikanásobných přenosech magnetizace. Štěpení signálu vlivem jednovazebné interakce uhlík-uhlík je odstraněno pomozí vývoje v konstantním čase nebo selektivní refokusací. Citrlivost navrhovaných experimentů dovoluje naměřit spektra 0.5 mM 25-meru RNA za čtyři hodiny nebo méně. Experimenty jsou aplikovatelné i na vzorky v orietovaných fázích.

Links

• LN00A016, research and development project: Name: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center