Detailed Information on Publication Record
2004
Identification and mapping of resistance genes to powdery mildew in barley.
ŘEPKOVÁ, Jana, Zdeňka KYJOVSKÁ, Pavel LÍZAL, Antonín DREISEITL, Ahmed JAHOOR et. al.Basic information
Original name
Identification and mapping of resistance genes to powdery mildew in barley.
Name in Czech
Identifikace a genetické mapování genů odolnosti k padlí travnímu u ječmene.
Authors
ŘEPKOVÁ, Jana (203 Czech Republic, guarantor), Zdeňka KYJOVSKÁ (203 Czech Republic), Pavel LÍZAL (203 Czech Republic), Antonín DREISEITL (203 Czech Republic) and Ahmed JAHOOR (208 Denmark)
Edition
2004. vyd. Praha, Czech Journal of Genetics and Plant Breeding, p. 51-51, 1 pp. 2004
Publisher
Czech academy of agricultural science
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/04:00011797
Organization unit
Faculty of Science
ISSN
Keywords in English
Barley; Hordeum vulgare; powdery mildew; gene mapping; microsatellite
Změněno: 17/7/2008 08:26, prof. RNDr. Jana Řepková, CSc.
V originále
The introduction of resistance genes to powdery mildew (Blumeria graminis f. sp. hordei) present in two accessions (PI354949 and PI466495) of wild barley (Hordeum vulgare subsp. spontaneum) into H. vulgare was performed by the cross with the variety Tiffany. Powdery mildew resistance tests on plants of F2 generation revealed the segregation ratio 15 : 1 in Tiffany x PI354949 and 3 : 1 in Tiffany x PI466495, which indicated presence of two and one dominant resistance genes, respectively. To identify individual R genes, technology of microsatellite DNA markers and linkage recombination analysis was applied. One of resistance genes in PI354949 was determined to be located in Mla locus linked with Bmac0213 (25.0 cM in distal position) on chromosome 1H and the other on chromosome 7H linked with Bmag0507 (14.0 cM in proximal position). In PI466495, R gene was linked with Bmac0213 (8.4 cM in distal position) and it is also located in Mla locus. The perspective aim of this work is fine-mapping of the promising gene located on 7H chromosome and identification of tightly linked DNA markers for marker assisted selection.
In Czech
Byly identifikovány dva zdroje odolnosti k padlí travnímu (Blumeria graminis f. sp. hordei) u ječmene (Hordeum vulgare subsp. spontaneum). Křížení těchto zdrojů s odrůdou Tiffany (Hordeum vulgare) umožnilo identifikovat v generaci F2 štěpný poměr 15 : 1 u Tiffany x PI354949 a 3 : 1 u Tiffany x PI466495, což odpovídá determinaci odolnosti jedním a dvěma geny. Mikrosatelitové DNA markery a rekombinační analýza v F2 byly využity k lokalizaci genů odolnosti do genetické mapy.
Links
| GA522/03/0112, research and development project |
|