D 2004

Gene expression in colorectal carcinoma determined by cDNA microarrays using a combination of several evaluation approaches.

JANSOVÁ, Eva, Petr KRONTORÁD, Zbyněk SVOBODA, Irena KOUTNÁ, Tomáš PAVLÍK et. al.

Basic information

Original name

Gene expression in colorectal carcinoma determined by cDNA microarrays using a combination of several evaluation approaches.

Name in Czech

Exprese genů u kolorektálního karcinomu určená pomocí cDNA mikročipů užitím kombinace několika vyhodnocovacích postupů

Authors

JANSOVÁ, Eva (203 Czech Republic), Petr KRONTORÁD (203 Czech Republic), Zbyněk SVOBODA (203 Czech Republic), Irena KOUTNÁ (203 Czech Republic), Tomáš PAVLÍK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Marie JAROŠOVÁ (203 Czech Republic), Jan ŽALOUDÍK (203 Czech Republic) and Stanislav KOZUBEK (203 Czech Republic, guarantor)

Edition

First Edition 2004. Brno, Biophysics of the Genome, p. 29-37, 9 pp. 2004

Publisher

Masaryk University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/04:00009700

Organization unit

Faculty of Informatics

ISBN

80-210-3560-9

Keywords in English

microarrays

Tags

Tags

International impact
Změněno: 29/10/2010 14:34, prof. RNDr. Michal Kozubek, Ph.D.

Abstract

ORIG CZ

V originále

We have compared colorectal carcinoma tissues with parallel samples of epithelial tissue and identified, by means of Human 1.7K cDNA microarrays, a set of genes with significantly altered expression in 12 patients. We used a combination of several approaches of microarray data analysis to be sure that the results were reliable. Although we have found differences between the results obtained by various image analysis software packages and using different statistical tools, we have identified the group of 22 genes with significantly altered expression in colorectal carcinoma tissue as compared with epithelial tissue using all evaluation approaches. Hierarchical clustering showed the possibility of dividing the patients into two groups according to the presence of metastases in regional lymph nodes. We have proposed 6 genes as potential markers for the detection of the presence of regional metastases in patients. We suppose that the proposed advance in the analysis of microarray data could lead to easier, quicker and more effective diagnostics of various diseases dependent on changes in the expression profile.

In Czech

Exprese genů u kolorektálního karcinomu určená pomocí cDNA mikročipů užitím kombinace několika vyhodnocovacích postupů.

Links

AV0Z5004920, plan (intention)
Name: Biofyzikální vlastnosti živých systémů a jejich změny vlivem faktorů vnějšího prostředí
GA202/02/0804, research and development project
Name: Určení radiačního rizika pro vznik chronické myeloidní leukémie na základě měření vzdáleností mezi geny ABL a BCR v hematopoietických buňkách
Investor: Czech Science Foundation, Chronic myeloid leukemia radiation risk estimation based on BCR-ABL distances in hemotopoietic cells
GA202/04/0907, research and development project
Name: Cytometrie s vysokým rozlišením na živých buňkách
Investor: Czech Science Foundation, High-resolution cytometry of living cells
IAA5004306, research and development project
Name: Struktura lidského genomu
Investor: Academy of Sciences of the Czech Republic, Human genome structure
KSK5052113, research and development project
Name: Struktura, exprese a interakce genomu
ME 565, research and development project
Name: Struktura a funkce interfázních chromosomů
MSM 143300002, plan (intention)
Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NC6987, research and development project
Name: Epigeneticky kontrolované změny exprese genů u nádorových onemocnění
Investor: Ministry of Health of the CR, Epigenetic control of gene expression in malignant diseases