Detailed Information on Publication Record
2004
Dynamics of HP1 transgene loci movement and silencing in living cells
ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Pavel MATULA, Petr MATULA et. al.Basic information
Original name
Dynamics of HP1 transgene loci movement and silencing in living cells
Name in Czech
Dynamika pohybu a klidového stavu HP1 transgenních lokusů v živých buňkách
Authors
ONDŘEJ, Vladan (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic, guarantor), Emilie LUKÁŠOVÁ (203 Czech Republic), Pavel MATULA (203 Czech Republic), Petr MATULA (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic)
Edition
First Edition 2004. Brno, Biophysics of the Genome, p. 66-71, 6 pp. 2004
Publisher
Masaryk University
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14330/04:00009705
Organization unit
Faculty of Informatics
ISBN
80-210-3560-9
Keywords in English
cytometry
Tags
Tags
International impact
Změněno: 6/8/2010 14:58, doc. RNDr. Petr Matula, Ph.D.
V originále
Intranuclear localization and mobility of Cy3 labelled transgene loci were studied in living cells. Observations showed occurrence of several transgene copies in each cell nucleus. The majority of copies were localized in the centromeric heterochromatin defined by HP1beta-GFP fusion protein, the minority of copies in euchromatin. The tracking of loci showed restricted diffusive motion of these in the short-time range. During long-time observation of HP1beta domains and transgene loci, we have found that in most cases the proximity of these objects decreased within time. The changes of positions of HP1 domains were very small but transgene loci displayed directional movement towards the relevant HP1 domain. Our data records support the idea that the cell nucleus consists of several separated higher-order compartments. The genes relocate within these compartments, which is finally connected with changes of their expression status. In our experiments, the transgene loci were strongly silenced. Three days after the transfection of cells, their expression was reduced dramatically. At that time we found colocalization of transgene loci with HP1beta domains and centromeres. Moreover, the loci were methylated, which was confirmed using antibodies against trimethylated histone H3 at Lys9.
In Czech
Dynamika pohybu a klidového stavu HP1 transgenních lokusů v živých buňkách.
Links
| AV0Z5004920, plan (intention) |
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| IAA1065203, research and development project |
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| IAA5004306, research and development project |
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| IBS5004010, research and development project |
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| 1A8241, research and development project |
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