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@inproceedings{563805,
author = {Ondřej, Vladan and Kozubek, Stanislav and Lukášová, Emilie and Matula, Pavel and Matula, Petr and Kozubek, Michal},
address = {Brno},
booktitle = {Biophysics of the Genome},
edition = {First Edition 2004},
keywords = {cytometry},
language = {eng},
location = {Brno},
isbn = {80-210-3560-9},
pages = {66-71},
publisher = {Masaryk University},
title = {Dynamics of HP1 transgene loci movement and silencing in living cells},
year = {2004}
}
TY - JOUR
ID - 563805
AU - Ondřej, Vladan - Kozubek, Stanislav - Lukášová, Emilie - Matula, Pavel - Matula, Petr - Kozubek, Michal
PY - 2004
TI - Dynamics of HP1 transgene loci movement and silencing in living cells
PB - Masaryk University
CY - Brno
SN - 8021035609
KW - cytometry
N2 - Intranuclear localization and mobility of Cy3 labelled transgene loci were studied in living cells. Observations showed occurrence of several transgene copies in each cell nucleus. The majority of copies were localized in the centromeric heterochromatin defined by HP1beta-GFP fusion protein, the minority of copies in euchromatin. The tracking of loci showed restricted diffusive motion of these in the short-time range. During long-time observation of HP1beta domains and transgene loci, we have found that in most cases the proximity of these objects decreased within time. The changes of positions of HP1 domains were very small but transgene loci displayed directional movement towards the relevant HP1 domain. Our data records support the idea that the cell nucleus consists of several separated higher-order compartments. The genes relocate within these compartments, which is finally connected with changes of their expression status. In our experiments, the transgene loci were strongly silenced. Three days after the transfection of cells, their expression was reduced dramatically. At that time we found colocalization of transgene loci with HP1beta domains and centromeres. Moreover, the loci were methylated, which was confirmed using antibodies against trimethylated histone H3 at Lys9.
ER -
ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Pavel MATULA, Petr MATULA and Michal KOZUBEK. Dynamics of HP1 transgene loci movement and silencing in living cells. In \textit{Biophysics of the Genome}. First Edition 2004. Brno: Masaryk University, 2004, p.~66-71. ISBN~80-210-3560-9.
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