J 2004

Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy

KOZUBEK, Michal, Petr MATULA, Pavel MATULA and Stanislav KOZUBEK

Basic information

Original name

Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy

Name in Czech

Automatické snímání a zpracování multidimenzionálních obrazových dat v konfokální in vivo mikroskopii

Authors

KOZUBEK, Michal (203 Czech Republic, guarantor), Petr MATULA (203 Czech Republic), Pavel MATULA (203 Czech Republic) and Stanislav KOZUBEK (203 Czech Republic)

Edition

Microscopy research and technique, USA, Wiley-Liss, 2004, 1059-910X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.609

RIV identification code

RIV/00216224:14330/04:00009712

Organization unit

Faculty of Informatics

UT WoS

000223634800011

Keywords in English

automated microscopy; confocal microscopy; in vivo imaging; Nipkow disk

Tags

International impact, Reviewed
Změněno: 6/5/2009 12:37, doc. RNDr. Petr Matula, Ph.D.

Abstract

V originále

The successful development of visualization techniques for live cell imaging leads to the development of suitable software for the acquisition and processing of multidimensional image data. This report compares several possible approaches to image acquisition and processing in confocal in vivo microscopy and suggests new alternatives to the published methods. Special attention is paid to spinning disk systems based either on a classical Nipkow disk or on the microlens principle. This study shows how to optimize image acquisition process in live cell studies using camera binning feature and how to perform object tracking using a new fast image registration method based on the graph theory.

In Czech

Práce se zabývá automatickým snímáním a zpracováním multidimenzionálních obrazových dat v konfokální in vivo mikroskopii.

Links

GA202/04/0907, research and development project
Name: Cytometrie s vysokým rozlišením na živých buňkách
Investor: Czech Science Foundation, High-resolution cytometry of living cells
IAA5004306, research and development project
Name: Struktura lidského genomu
Investor: Academy of Sciences of the Czech Republic, Human genome structure
IBS5004010, research and development project
Name: Vývoj nových diagnostických technik pro onkologii
Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)
Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy