Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry

KOZUBEK, Michal, Petr MATULA and Pavel MATULA. Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry. In International Symposium on Biomedical Imaging. Arlington, USA: IEEE, 2004, p. 69-72. ISBN 0-7803-8389-3.

Basic information

Original name

Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry

Name in Czech

Automatizovaná konfokální mikroskopie: Způsob dosažení jak kvality tak kvantity v 3D obrazové cytometrii

Authors

KOZUBEK, Michal (203 Czech Republic, guarantor), Petr MATULA (203 Czech Republic) and Pavel MATULA (203 Czech Republic)

Edition

Arlington, USA, International Symposium on Biomedical Imaging, p. 69-72, 4 pp. 2004

Publisher

IEEE

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/04:00009713

Organization unit

Faculty of Informatics

ISBN

0-7803-8389-3

UT WoS

000227671300018

Keywords in English

image cytometry; confocal microscopy

Tags

cbia-web, confocal microscopy, image cytometry

Tags

International impact, Reviewed

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Abstract

V originále

Image cytometry is mostly associated with high quality but low quantity, whereas flow cytometry is associated with high quantity but low quality. Achieving both quality and quantity in cytometry is possible in the case of automated confocal microscopy systems. We have shown that the automation of image acquisition and image processing in confocal microscopy is possible for specific tasks such as 2D and 3D FISH imaging. This paper is focused on practical problems and solutions that we have encountered during our research. We believe that our experience might be helpful to all those who decide to automate their light microscopy systems for cell studies, i.e. for image cytometry purposes.

In Czech

Článek pojednává o automatizované konfokální mikroskopii a způsobu dosažení jak kvality, tak kvantity v 3D obrazové cytometrii

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Links

IAA5004306, research and development project	Name: Struktura lidského genomu Investor: Academy of Sciences of the Czech Republic, Human genome structure
IBS5004010, research and development project	Name: Vývoj nových diagnostických technik pro onkologii Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)	Name: Využití počítačové analýzy obrazu v optické mikroskopii Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy