Detailed Information on Publication Record
2004
Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
AMRICHOVÁ, Jana, Emilie LUKÁŠOVÁ, Miroslav VAŘECHA, Stanislav KOZUBEK, Vladan ONDŘEJ et. al.Basic information
Original name
Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Name in Czech
Topografická in situ a in vivo charakterizace telomeru stanovená ve zdravých a v různých typech nádorových buněčných liniích
Authors
AMRICHOVÁ, Jana (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Miroslav VAŘECHA (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Vladan ONDŘEJ (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, guarantor)
Edition
Abstracts of the Congress of International Society for Analytical Cytology, Cytometry 59A, p.91, 2004
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.698
RIV identification code
RIV/00216224:14330/04:00009715
Organization unit
Faculty of Informatics
ISSN
UT WoS
000221319900197
Keywords in English
Telomere
Tags
Tags
International impact, Reviewed
Změněno: 30/10/2010 11:24, prof. RNDr. Michal Kozubek, Ph.D.
V originále
Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied using repeated fluorescence in situ hybridization, high-resolution image cytometry and three-dimensional image analysis of human blood lymphocytes before and after stimulation (Amrichová et al., 2003). Our results suggest that in both G0- and stimulated lymphocytes, centromeres of HSA 3, 8, 9, and 13 are predominantly localized at the periphery of the cell nucleus; only centromere of HSA 19 in G0-lymphocytes is localized more centrally. Both p- and q-telomeres mostly adopt positions in the nuclear interior; q-telomeres are usually nearer to the center of the cell nucleus as compared to p-telomeres. Using repeated hybridization, tethering between telomeres of heterologous chromosomes 8, 9, and 19 was investigated. An attempt was made to find which particular telomeres are involved in telomere associations and whether any associations can be found also between homologous telomeres. We found no tethering of heterologous telomeres of chromosomes 8, 9, and 19. In contrary, both pairs of homologous telomeres of chromosome 19 (but not in other chromosomes) are tethered (associated) very frequently (Amrichová et al., 2003). Our interest was extended by investigation of topography of telomeres and incidence of telomere-association phenomenon in various types of tumor cells in situ and in vivo. Results of our observations will be discussed
In Czech
Topografická in situ a in vivo charakterizace telomeru stanovená ve zdravých a v různých typech nádorových buněčných liniích.
Links
GA202/02/0804, research and development project |
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GA301/01/0186, research and development project |
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IAA5004306, research and development project |
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IBS5004010, research and development project |
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ME 565, research and development project |
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MSM 143300002, plan (intention) |
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NC6987, research and development project |
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