VAŘECHA, Miroslav, Jana AMRICHOVÁ, Vladan ONDŘEJ, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK and Michal KOZUBEK. Automated in vivo imaging of cell interior using fluorescent proteins. In Abstracts of the Congress of International Society for Analytical Cytology, Cytometry 59A, p.107. 2004. ISSN 0196-4763.
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Basic information
Original name Automated in vivo imaging of cell interior using fluorescent proteins
Name in Czech Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů
Authors VAŘECHA, Miroslav (203 Czech Republic), Jana AMRICHOVÁ (203 Czech Republic), Vladan ONDŘEJ (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, guarantor).
Edition Abstracts of the Congress of International Society for Analytical Cytology, Cytometry 59A, p.107, 2004.
Other information
Original language English
Type of outcome Conference abstract
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.698
RIV identification code RIV/00216224:14330/04:00010822
Organization unit Faculty of Informatics
ISSN 0196-4763
UT WoS 000221319900243
Keywords in English Telomere
Tags telomere
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 30/10/2010 11:22.
Abstract
Techniques with fluorescent proteins have become very valuable tools for the study of cellular processes in living cells. These proteins enable the non-invasive quantitative visualization in vivo as molecular tags on natively non-fluorescent molecules of interest. Fluorescent proteins are welcome innovation in the field of cell biology, because in vivo experiments bring new interesting results and different point of view than in vitro and in situ experiments. This presentation will address automated 2D/3D high-resolution confocal image acquisition and analysis of cells stained with fluorescent proteins. While the approaches to automated in situ imaging of cell interior have been previously described by our group (Cytometry 45, 1-12, 2001), this contribution will concentrate on the modifications (both hardware and software ones) of our high-resolution image cytometers made for the purpose of automated in vivo imaging.
Abstract (in Czech)
Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů.
Links
GP204/03/D031, research and development projectName: Apoptózu vyvolávající faktor (AIF): Jeho uvolnění z mitochondrie a změny, které vyvolává ve struktuře jaderného chromatinu
Investor: Czech Science Foundation, Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin
MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
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