Biochemical analysis of soil organic matter and microbial biomass composition - a pilot study

HOFMAN, Jakub and Ladislav DUŠEK. Biochemical analysis of soil organic matter and microbial biomass composition - a pilot study. European Journal of Soil Biology. vol. 39, No 1, p. 217-224. ISSN 1164-5563. 2003.

Basic information

• Original name: Biochemical analysis of soil organic matter and microbial biomass composition - a pilot study
• Name in Czech: Biochemická analýza složení půdní organické hmoty a mikrobiální biomasy - pilotní studie
• Authors: HOFMAN, Jakub (203 Czech Republic, guarantor) and Ladislav DUŠEK (203 Czech Republic).
• Edition: European Journal of Soil Biology, 2003, 1164-5563.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 40104 Soil science
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.830
• RIV identification code: RIV/00216224:14310/03:00010826
• Organization unit: Faculty of Science
• UT WoS: 000187082900006
• Keywords in English: soil microbial biomass
• Tags: soil microbial biomass
• Tags: International impact, Reviewed

Abstract

Biochemical composition of both intracellular (biomass) and extracellular soil organic matter was determined after extraction with 0.5 M K2SO4. Extractable carbon, hexoses, pentoses, total reducing sugars, ninhydrin-reactive nitrogen, proteins and DNA content were colorimetrically determined. The objective of the pilot study was to examine the information potential included in newly measured biochemical characteristics, their environmental variance and the relationships with main soil properties. Correlation analysis and PCA showed independence between biochemical parameters and physico-chemical properties of the soil. Thus, the parameters characterising biochemical composition of the soil biomass and extracellular matter seem to bring new information about the soils beyond the physico-chemical parameters. They also seem to reveal a more detailed view on microbial biomass or extracellular organic matter pool than Cbio or Cext alone, respectively. The variance which occurred in biochemical characteristics displayed also a high discrimination potential between the defined soil categories. Three types of indices were newly proposed: index I ("substrate quantity index") - the biomass-specific amount of the extracellular organic compounds, index II ("immobilisation ratio") - the portion of the organic compound immobilised in microbial biomass, and index III ("substrate quality index") - the extracellular organic compound content related to extracellular organic carbon. The indices displayed a higher potential than both soil biotic and abiotic parameters to discriminate soil characters and soil types.

Abstract (in Czech)

Biochemická analýza složení půdní organické hmoty a mikrobiální biomasy - pilotní studie

Links

• GA526/98/1147, research and development project: Name: Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)

Investor: Czech Science Foundation, Diversity and functional analysis of soil microbial communities in terrestrial ecosystems polluted by persistent organic pollutants (POPs)

• MSM 141100003, plan (intention): Name: Zevní prostředí - karcinogeneze - onkologie

Investor: Ministry of Education, Youth and Sports of the CR, Environment - carcinogenesis - oncology

• SI/340/1/00, research and development project: Name: Region specifický přístup v analýze ekologických rizik - metodika zhodnocení ve vztahu k přírodním katastrofám.