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@article{564541,
author = {Lukášová, Emilie and Kořístek, Zdeněk and Falk, Martin and Kozubek, Stanislav and Grigoryev, Sergei and Kozubek, Michal and Ondřej, Vladan and Kroupová, Iva},
article_location = {New York},
article_number = {1},
keywords = {human granulocytes differentiation; chromatin condensation; heterochromatin; HP1 proteins},
language = {eng},
issn = {0741-5400},
journal = {Journal of Leukocyte Biology},
title = {Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities},
volume = {77},
year = {2005}
}
TY - JOUR
ID - 564541
AU - Lukášová, Emilie - Kořístek, Zdeněk - Falk, Martin - Kozubek, Stanislav - Grigoryev, Sergei - Kozubek, Michal - Ondřej, Vladan - Kroupová, Iva
PY - 2005
TI - Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities
JF - Journal of Leukocyte Biology
VL - 77
IS - 1
SP - 100-111
EP - 100-111
PB - Society for Leukocyte Biology
SN - 07415400
KW - human granulocytes differentiation
KW - chromatin condensation
KW - heterochromatin
KW - HP1 proteins
N2 - We show that common heterochromatin antigenic protein markers [HP1alpha, -betta, -gamma and mono-, di-, and trimethylated histone H3 lysine 9 (H3K9)], although present in human blood progenitor CD34+ cells, differentiated lymphocytes, and monocytes, are absent in neutrophil granulocytes and to large extent, in eosinophils. Monomethylated and in particular, dimethylated H3K9 are present to variable degrees in the granulocytes of chronic myeloid leukemia (CML) patients, without being accompanied by HP1 proteins. In patients with an acute phase of CML and in acute myeloid leukemia patients, strong methylation of H3K9 and all isoforms of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression, and modality of treatment were observed. Histone methylation was found even in "cured" patients without Philadelphia chromosome (Ph) resulting from +(9;22)(q34;q11) BCR/ABL translocation, suggesting an incomplete process of developmentally regulated chromatin remodeling in the granulocytes of these patients. Similarly, reprogramming of leukemia HL-60 cells to terminal differentiation by retinoic acid does not eliminate H3K9 methylation and the presence of HP1 isoforms from differentiated granulocytes. Thus, our study shows for the first time that histone H3 methylation may be changed dramatically during normal cell differentiation. The residual histone H3 methylation in myeloid leukemia cells suggests an incomplete chromatin condensation that may be linked to the leukemia cell proliferation and may be important for the prognosis of disease treatment and relapse.
ER -
LUKÁŠOVÁ, Emilie, Zdeněk KOŘÍSTEK, Martin FALK, Stanislav KOZUBEK, Sergei GRIGORYEV, Michal KOZUBEK, Vladan ONDŘEJ and Iva KROUPOVÁ. Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities. \textit{Journal of Leukocyte Biology}. New York: Society for Leukocyte Biology, 2005, vol.~77, No~1, p.~100-111. ISSN~0741-5400.
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