KRAMÁŘOVÁ, Eva and Jiří SLANINA. ELICITATION OF SYNTHETIC ABILITIES OF IN VITRO CULTURES SCHIZANDRA CHINENSIS (TURCZ.) BAILL. In Book of Abstracts, XXXIV annual meeting ESNA. první. Novi Sad, Serbia and Monte Negro: ESNA, 2004, p. 214. ISBN 86-7520-033-1.
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Basic information
Original name ELICITATION OF SYNTHETIC ABILITIES OF IN VITRO CULTURES SCHIZANDRA CHINENSIS (TURCZ.) BAILL.
Name in Czech Elicitace syntetických schopností Schisandra chinensis v in vitro kulturách
Authors KRAMÁŘOVÁ, Eva (203 Czech Republic) and Jiří SLANINA (203 Czech Republic, guarantor).
Edition první. Novi Sad, Serbia and Monte Negro, Book of Abstracts, XXXIV annual meeting ESNA, p. 214-214, 2004.
Publisher ESNA
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 40100 4.1 Agriculture, Forestry, and Fisheries
Country of publisher Serbia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/04:00011932
Organization unit Faculty of Medicine
ISBN 86-7520-033-1
Keywords in English elicitation; plant cell culture; Schisandra chinensis; methyljasmonate; calli culture
Tags calli culture, elicitation, methyljasmonate, plant cell culture, Schisandra chinensis
Changed by Changed by: Mgr. Jiří Slanina, Ph.D., učo 689. Changed: 21/1/2005 14:33.
Abstract
Synthetic abilities of calli culture Schizandra chinensis in vitro have been studied in our studies. An elektrospray high-performance liquid chromatography and mass spectrometry interface coupled with a photodiode-array detector has been applied to the identification of a number of lignan constituents of SFE extracts the calli cultures Schisandra chinensis: gomisin J, gomisin N, schisanhenol, gomisin M, schisantherin B, deoxyschisandrin, gomisin R, gama-schisandrin and schisandrin. The calli cultures extracted from stalks of plants grown on Gamborg B5 medium with addition of PEG /polyethyenglykol Sigma 6000/ contained ( % in dry matter): schizandrin 4 times, gomisin 10 times and deoxyschizandrin 1,5 times higher volume in comparison to the control calli cultures. Antioxidative abilities /on the basis of reaction with DPPH radical/ of calli cultures gained from fruits were approximately four times higher compared to the calli cultures gained from stalks. By using methyljasmonate as elicitor in concentration 0,002 mg/l the volume of five lignans in calli cultures gained from stalks rose ten times compared to the control calli cultures. Calli and suspension cultures extracted from stalk posses higher acceptance of elicitor intervention than calli and suspension cultures extracted from fruit.
Abstract (in Czech)
Elicitace syntetických schopností Schisandra chinensis v in vitro kulturách byla sledována v naší studii.
Links
GA521/02/1129, research and development projectName: Využití biotechnologických metod k produkci sekundárních metabolitů, šlechtění a mikropropagaci léčivé rostliny klanoprašky čínské
Investor: Czech Science Foundation, The use of biotechnological methods in secondary metabolite production, breeding and micropropagation of medical plant Schizandra chinensis
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