Detailed Information on Publication Record
2004
Changes of [Ca2+] in the t-tubule lumen during activity may modulate the inotropic state of rat cardiac ventricular myocytes
PÁSEK, Michal, Georges CHRISTÉ, Jiří ŠIMURDA and Clive ORCHARDBasic information
Original name
Changes of [Ca2+] in the t-tubule lumen during activity may modulate the inotropic state of rat cardiac ventricular myocytes
Name in Czech
Změny [Ca2+] uvnitř t-tubulů mohou měnit inotropní stav srdečních ventrikulárních buněk potkana
Authors
PÁSEK, Michal (203 Czech Republic, guarantor), Georges CHRISTÉ (250 France), Jiří ŠIMURDA (203 Czech Republic) and Clive ORCHARD (203 Czech Republic)
Edition
Meeting of The Physiological Society (Oxford), 2004
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10610 Biophysics
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14110/04:00011947
Organization unit
Faculty of Medicine
Keywords in English
cardiac cell inotropy; tubular system; quantitative modelling
Tags
International impact, Reviewed
Změněno: 2/1/2010 11:17, doc. Ing. Michal Pásek, Ph.D.
V originále
The majority of trans-sarcolemmal Ca2+ flux occurs across the t-tubule membrane, which therefore plays a key role in cardiac excitation-contraction coupling; however Ca2+ within the t-tubules does not equilibrate instantaneously with the bulk extracellular solution. Thus, during activity, changes in [Ca2+] may occur within the t-tubule lumen, which would, in turn, alter trans-sarcolemmal Ca2+ flux. To test this hypothesis, we used a computer model of rat ventricular myocyte including a t-tubular system. The action potentials in the two membranes were not significantly different, reflecting the electrical coupling of the two compartments. However, [Ca2+] within the t-tubule lumen decreased on each stimulus, and decreased cumulatively, until reaching a new dynamic steady state, with increasing stimulation rate. These data suggest that activity-dependent depletion of Ca2+ within the t-tubule lumen, adjacent to the trans-sarcolemmal Ca2+ flux pathways, decreases the Ca load, and hence lowers the inotropic status, of ventricular myocytes.
In Czech
Práce prezentuje výsledky simulací na modelu srdeční komorové buňky potkana ukazující vliv koncentračních změn tubulárních iontů Ca2+ (během buněčné aktivity) na intracelulární vápníkovou rovnováhu a inotropní stav komorových buněk.
Links
| AV0Z2076919, plan (intention) |
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| GP204/02/D129, research and development project |
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