ČECHOVÁ, Leona, Eva DURNOVÁ, Silvie ŠIKUTOVÁ, Jiří HALOUZKA and Miroslav NĚMEC. Characterization of spirochetal isolates from arthrops collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis. Journal of Chromatography B. 2004, 808/2004, No 2, p. 249-254. ISSN 1570-0232. |
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@article{565510, author = {Čechová, Leona and Durnová, Eva and Šikutová, Silvie and Halouzka, Jiří and Němec, Miroslav}, article_number = {2}, keywords = {Spirochetes;Arthropods;Fatty acid methyl esters}, language = {eng}, issn = {1570-0232}, journal = {Journal of Chromatography B}, title = {Characterization of spirochetal isolates from arthrops collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis}, volume = {808/2004}, year = {2004} }
TY - JOUR ID - 565510 AU - Čechová, Leona - Durnová, Eva - Šikutová, Silvie - Halouzka, Jiří - Němec, Miroslav PY - 2004 TI - Characterization of spirochetal isolates from arthrops collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis JF - Journal of Chromatography B VL - 808/2004 IS - 2 SP - 249-254 EP - 249-254 SN - 15700232 KW - Spirochetes;Arthropods;Fatty acid methyl esters N2 - Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one "Spironema culicis" strain recovered from mosquito Culex pipiens and seven spirochetal strains (non identified) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography columm in conjuction with Microbial Identification System Sherlock (MIDI Inc.,Newark, DE,USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B.burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool preliminary identification of spirochetes isolated from ticks and dipterans. ER -
ČECHOVÁ, Leona, Eva DURNOVÁ, Silvie ŠIKUTOVÁ, Jiří HALOUZKA and Miroslav NĚMEC. Characterization of spirochetal isolates from arthrops collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis. \textit{Journal of Chromatography B}. 2004, 808/2004, No~2, p.~249-254. ISSN~1570-0232.
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