Detailed Information on Publication Record
2004
Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection
KLEJDUS, Borivoj, Jan VACEK, Vojtech ADAM, Josef ZEHNÁLEK, René KIZEK et. al.Basic information
Original name
Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection
Name in Czech
Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí
Authors
KLEJDUS, Borivoj (203 Czech Republic), Jan VACEK (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), René KIZEK (203 Czech Republic), Libue TRNKOVÁ (203 Czech Republic, guarantor) and Vlastimil KUBÁN (203 Czech Republic)
Edition
Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2004, 1570-0232
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.176
RIV identification code
RIV/00216224:14310/04:00011226
Organization unit
Faculty of Science
UT WoS
000221945700004
Keywords in English
Isoflavones; HPLC; electrochemical detection
Změněno: 15/2/2005 13:34, prof. RNDr. Libuše Trnková, CSc.
V originále
A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE) by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 02 min 87%; 22 min 60%; 27 min 50%; 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450mV was chosen for our purposes. Calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1.9 pg. Isoflavones extracted from soybean products (farina, meat, milk) by the accelerated solvent extraction (ASE) procedure and isoflavones present in human urine were determined the HPLC-ED method.
In Czech
Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí
Links
| GA203/02/0422, research and development project |
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| IAA1163201, research and development project |
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