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@article{567244,
author = {Šebela, Marek and Kopečný, David and Lamplot, Zbyněk and Havliš, Jan and Thomas, Henrik and Shevchenko, Andrej},
keywords = {amine oxidase; cyclodextrin; matrix-assisted laser-desorption ionizationtime-of-flight (MALDITOF); mass spectrometry (MS); protein modification; thermostability.},
language = {eng},
issn = {0885-4513},
journal = {Biotechnology and Applied Biochemistry},
title = {Thermostable -cyclodextrin conjugates of two similar plant amine oxidases and their properties},
volume = {41},
year = {2005}
}
TY - JOUR
ID - 567244
AU - Šebela, Marek - Kopečný, David - Lamplot, Zbyněk - Havliš, Jan - Thomas, Henrik - Shevchenko, Andrej
PY - 2005
TI - Thermostable -cyclodextrin conjugates of two similar plant amine oxidases and their properties
JF - Biotechnology and Applied Biochemistry
VL - 41
SP - 77-84
EP - 77-84
SN - 08854513
KW - amine oxidase
KW - cyclodextrin
KW - matrix-assisted laser-desorption ionizationtime-of-flight (MALDITOF)
KW - mass spectrometry (MS)
KW - protein modification
KW - thermostability.
N2 - Syntheses of conjugates of garden pea (Pisum sativum) and grass pea (Lathyrus sativus) amine oxidases (PSAO and GPAO respectively) with BCD (-cyclodextrin), performed to improve the thermostability of the enzymes, are described in the present study. Periodateoxidized BCD reacted with the enzyme proteins via free primary amino groups in a buffered solution containing cyanoborohydride as a reductant. Although the specific activities of PSAO and GPAO partially decreased after modification, Km values determined for the best diamine substrates remained almost unchanged. Both the BCD conjugates could be incubated at 65 C for 30 min without considerable inactivation, and the residual activity remained detectable even after incubation at 75 C. The conjugates contained approx. 30% of neutral sugars. Molecular masses of BCD PSAO and BCDGPAO (180 kDa), as estimated by gelpermeation chromatography, were higher compared with the value of 145 kDa for the native enzymes. This was in good correlation with the number of modified lysine residues determined by a spectrophotometric method. Peptide mass fingerprints of tryptic digests of BCDPSAO and BCDGPAO were less specific than those of the native enzymes when compared with the database sequence of PSAO. As a consequence of the modification, many unidentified peaks were observed in the digests of the studied conjugates that were not seen in the digests of native PSAO and GPAO. Only some of these peaks overlapped between BCD PSAOand BCDGPAO. The BCD conjugates described in the present study represent suitable candidates for biotechnological applications, e.g. in analyses using biosensors, which might benefit from increased storage stability and amine oxidation at high temperatures.
ER -
ŠEBELA, Marek, David KOPEČNÝ, Zbyněk LAMPLOT, Jan HAVLIŠ, Henrik THOMAS a Andrej SHEVCHENKO. Thermostable -cyclodextrin conjugates of two similar plant amine oxidases and their properties. \textit{Biotechnology and Applied Biochemistry}. 2005, roč.~41, s.~77-84, 7 s. ISSN~0885-4513.
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