Detailed Information on Publication Record
2004
Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium
KLEJDUS, Boivoj, Josef ZEHNÁLEK, Vojtch ADAM, Jií PETEK, René KIZEK et. al.Basic information
Original name
Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium
Name in Czech
Využití vysokoúinné kapalinové chromatografie pro stanovení glutatiónu v kukuiných jádrech vystavených kadmiu
Authors
KLEJDUS, Boivoj (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), Vojtch ADAM (203 Czech Republic), Jií PETEK (203 Czech Republic), René KIZEK (203 Czech Republic), Jan VACEK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), Roland ROZIK (703 Slovakia), Ladislav HAVEL (203 Czech Republic) and Vlastimil KUBÁ (203 Czech Republic)
Edition
Analytica Chimica Acta, elsevier, 2004, 0003-2670
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.588
RIV identification code
RIV/00216224:14310/04:00011228
Organization unit
Faculty of Science
UT WoS
000223567500013
Keywords in English
Maize; Cadmium; Glutathione
Tags
Změněno: 20/6/2008 12:07, prof. RNDr. Libuše Trnková, CSc.
V originále
Changes in fresh weight, total protein amounts (Bradfords method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for 5 days at three different cadmium concentrations (0, 10 and 100 micromol /l CdCl2). A highly sensitive HPLC/MS method was used for the determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column. An isocratic mode with acetonitrile0.01% TFA (5:95, flow rate 0.1 ml min1 and 30 C) was applied.The m/z spectra and the data for the selected ion monitoring (SIM) mode were recorded at m/z for glutathione 308 - 179. Cadmium concentration was measured by a differential pulse adsorptive stripping voltammetry (DPASV) after deposition on a hanging mercury drop electrode (HMDE) at potential 0.7V (accumulation time 180 s, acetate buffer of pH 3.6, 22 C). An AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of the HMDE as a working electrode, an Ag/AgCl/3 mol l1 KCl as a reference electrode and a Pt-wire as an auxiliary electrode was employed. The maize kernels exposed to the highest cadmium concentration (100 micromol/l)germinated formerly and much better. A rapid increase of the fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd2+ ions in the germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with the exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
In Czech
Využití vysokoúinné kapalinové chromatografie ve spojení s hmotnostní spektrometrií pro stanovení glutatiónu v kukuiných jádrech vystavených kadmiu
Links
| GA203/02/0422, research and development project |
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| IAA1163201, research and development project |
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