KIZEK, René, Jan VACEK, Libuše TRNKOV, Bořivoj KLEJDUS and Ladislav HAVEL. Využití katalytických reakcí na rtuťových elektrodách pro elektrochemické stanovení metalothioneinu (Application of catalytic reactions on a mercury electrode for electrochemical detection of metallothioneins). Chemick Listy. Praha: esk spolenost chemick, 2004, vol. 98, No 2, p. 166 - 173. ISSN 0009-2770.
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Basic information
Original name Využití katalytických reakcí na rtuťových elektrodách pro elektrochemické stanovení metalothioneinu
Name in Czech Využití katalytických reakcí na rtuťových elektrodách pro elektrochemické stanovení metalothioneinu
Name (in English) Application of catalytic reactions on a mercury electrode for electrochemical detection of metallothioneins
Authors KIZEK, René (203 Czech Republic), Jan VACEK (203 Czech Republic), Libuše TRNKOV (203 Czech Republic, guarantor), Bořivoj KLEJDUS (203 Czech Republic) and Ladislav HAVEL (203 Czech Republic).
Edition Chemick Listy, Praha, esk spolenost chemick, 2004, 0009-2770.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study 10405 Electrochemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.348
RIV identification code RIV/00216224:14310/04:00011232
Organization unit Faculty of Science
UT WoS 000221682700003
Keywords in English metallothionein; electrochemical analysis; catalytic reactions; peak H; Brdicka reaction
Tags Brdicka reaction, catalytic reactions, electrochemical analysis, metallothionein, Peak H
Changed by Changed by: prof. RNDr. Libuše Trnková, CSc., učo 1027. Changed: 15/2/2005 20:16.
Abstract
Metallothioneins (MTs) belong to a group of oligo- and polypeptides which play an important role in the metabolism of metals in animals, plants and microorganisms. The metabolic function of MTs consists not only in detoxication of an organism but also in the homeostasis of essential metals. This paper reports a novel approach to electroanalysis of MTs. The electrochemical determination of MTs is based on catalytic processes which proceed at very negative potentials on mercury electrodes (from -1.7 V to -1.9 V vs. Ag/AgCl/KCl). These processes accompanied by evolution of hydrogen from supporting electrolyte components, include the Heyrovsky presodium wave and/or Brdicka reaction. It was found that SH groups present in MTs are responsible for catalytic processes. The catalytic signal of a MT at nanomolar concentrations can be detected on mercury electrodes using potentiostatic electrochemical methods. The highest sensitivity in the determination of MTs was observed with a galvanostatic method such as derivative potentiometric stripping analysis (CPSA) which produces the peak H. The coupling of CPSA with the adsorptive transfer stripping technique (AdTS) allows the determination of MTs at a femtomole level in a low amount of sample (5 ěl). Our recent results obtained by AdTS + CPSA show that detection limits for particular MTs can be improved by adding [Co(NH3)6] Cl3 to the sample analyzed. This is probably due to the formation of a complex between [Co(NH3)6] Cl3 and MT. We think that the peak H can be used in physiological studies of metal metabolism.
Abstract (in English)
Metallothioneins (MTs) belong to a group of oligo- and polypeptides which play an important role in the metabolism of metals in animals, plants and microorganisms. The metabolic function of MTs consists not only in detoxication of an organism but also in the homeostasis of essential metals. This paper reports a novel approach to electroanalysis of MTs. The electrochemical determination of MTs is based on catalytic processes which proceed at very negative potentials on mercury electrodes (from -1.7 V to -1.9 V vs. Ag/AgCl/KCl). These processes accompanied by evolution of hydrogen from supporting electrolyte components, include the Heyrovsky presodium wave and/or Brdicka reaction. It was found that SH groups present in MTs are responsible for catalytic processes. The catalytic signal of a MT at nanomolar concentrations can be detected on mercury electrodes using potentiostatic electrochemical methods. The highest sensitivity in the determination of MTs was observed with a galvanostatic method such as derivative potentiometric stripping analysis (CPSA) which produces the peak H. The coupling of CPSA with the adsorptive transfer stripping technique (AdTS) allows the determination of MTs at a femtomole level in a low amount of sample (5 ěl). Our recent results obtained by AdTS + CPSA show that detection limits for particular MTs can be improved by adding [Co(NH3)6] Cl3 to the sample analyzed. This is probably due to the formation of a complex between [Co(NH3)6] Cl3 and MT. We think that the peak H can be used in physiological studies of metal metabolism.
Links
GA203/02/0422, research and development projectName: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí
Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
IAA1163201, research and development projectName: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin
Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
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