Detailed Information on Publication Record
2004
Elimination voltammetry of oligonucleotides
TRNKOVÁ, Libuše, Jana TOPINKOVÁ, František JELEN and Irena POSTBIEGLOVÁBasic information
Original name
Elimination voltammetry of oligonucleotides
Name in Czech
Eliminační voltametrie oligonukleotidů
Authors
TRNKOVÁ, Libuše (203 Czech Republic, guarantor), Jana TOPINKOVÁ (203 Czech Republic), František JELEN (203 Czech Republic) and Irena POSTBIEGLOVÁ (203 Czech Republic)
Edition
1. vyd. Thessaloniki, Creece, Book of Abstracts I - 55th Annual Meeting of the ISE, p. 429 - 429, 1 pp. 2004
Publisher
International Society of Electrochemistry
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10405 Electrochemistry
Country of publisher
Greece
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/04:00011234
Organization unit
Faculty of Science
Keywords in English
Oligonucleotides; elimination voltammetry with linear scan; partial currents; reduction of adenine and cytosine
Změněno: 15/2/2005 21:08, prof. RNDr. Libuše Trnková, CSc.
V originále
Oligodeoxynucleotides (ODNs) containing adenines (A), cytosines (C) and guanines (G) are electroactive species and produce cathodic (A,C) and anodic (G) voltammetric peaks at mercury electrodes. The reduction peak potentials of A and C are located very close to each other, so that usually only one voltammetric peak is observed. One signal only can be observed even when adsorptive transfer stripping voltammetry (AdTSV) is used. The elimination voltammetry with linear scan (EVLS) used in connection with a hanging mercury electrode is able to distinguish between A and C signals. The EVLS is based on elimination of one or two selected currents from total currents measured by linear scan voltammetry. The function eliminating kinetic and charging currents, and conserving the diffusion current was calculated for the case of adsorbed electroactive substance, and the theoretical elimination curve has a peak-counterpeak form. Various elimination functions were applied in the analysis of oligonucleotides containing different contents of A, C and G, their sequences, and length of ODNs. The height and potential of EVLS signals were affected by pH, buffer components, content of bases, and time of accumulation. Our results showed that the voltammetric signal of the bases can be separated, and that EVLS in connection with AdTSV is a promising tool for qualitative and quantitative studies of not only homo-ODNs but also hetero-ODNs, including hairpins.
In Czech
Oligonukleotidy(ODNs) obsahující adenin (A), cytosin (C) a guanin (G) jsou elektroaktivní částice a produkují na rtuťové elektrodě katodické (A,C) a anodické (G) signály. Redukční píky A a C jsou potenciálově lokalizovány tak blízko, že splývají a běžnými elektrochemickými metodami jsme schopni registrovat signál jen jeden. Pomocí eliminační voltametrie s lineární polarizací elektrody(EVLS) se nám podařilo redukční píky A a C nejen rozlišit, ale i dokázat, že v kombinaci EVLS s adsorptvním strippingem lze uvažovat o rozlišení sekvence bází v jednotlivých ODNs.
Links
| GA203/02/0422, research and development project |
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| IAA1163201, research and development project |
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