Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode

VACEK, Jan, Libuše TRNKOVÁ, František JELEN and René KIZEK. Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode. In Book of Abstracts - 55th Annual Meeting of the ISE. 1st ed. Thessaloniki, Greece: International Society of Electrochemistry, 2004, p. 431 - 431.

Basic information

Original name

Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode

Name in Czech

Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod

Authors

VACEK, Jan (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), František JELEN (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

1. vyd. Thessaloniki, Greece, Book of Abstracts - 55th Annual Meeting of the ISE, p. 431 - 431, 1 pp. 2004

Publisher

International Society of Electrochemistry

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/04:00011235

Organization unit

Faculty of Science

Keywords in English

metallothioneines; phytochelatins; glutathione; mercury electrode;catalytic reactions; Brdicka reaction;peak H

Tags

Brdicka reaction, catalytic reactions, Glutathione, Mercury Electrode, metallothioneines, Peak H, phytochelatins

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Abstract

V originále

The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was successfully performed in a cobalt(III)-containing solution with ammonia buffer by differential pulse voltammetry (DPV). The CPSA (peak H) and DPV (Brdicka reaction) can be used in connection with the adsorptive transfer stripping (AdTS) technique, which is capable of determining nano/femtomole levels of metallothioneine, phytochelatin and glutathione in mL of the sample.

In Czech

Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod.

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Links

GA203/02/0422, research and development project	Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
IAA1163201, research and development project	Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.