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@inproceedings{567330,
author = {Klejdus, Bořivoj and Petrlová, Jitka and Potěšil, David and Trnková, Libuše and Mikelová, Radka and Zehnálek, Josef and Kizek, René and Kubáň, Vlastimil},
address = {Thessaloniki, Greece},
booktitle = {Book of Abstracts - 55th Annual Meeting of the ISE},
edition = {1},
keywords = {HPLC-ED; square wave voltammetry; isoflavones; daidzein; genistein; soybean products},
language = {eng},
location = {Thessaloniki, Greece},
pages = {433 - 433},
publisher = {International Society of Electrochemistry},
title = {Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)},
year = {2004}
}
TY - JOUR
ID - 567330
AU - Klejdus, Bořivoj - Petrlová, Jitka - Potěšil, David - Trnková, Libuše - Mikelová, Radka - Zehnálek, Josef - Kizek, René - Kubáň, Vlastimil
PY - 2004
TI - Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)
PB - International Society of Electrochemistry
CY - Thessaloniki, Greece
KW - HPLC-ED
KW - square wave voltammetry
KW - isoflavones
KW - daidzein
KW - genistein
KW - soybean products
N2 - A highly sensitive HPLC-ED was developed for the determination of isoflavones. The electrochemical behaviour of daidzein and genistein was studied on a carbon paste electrode by square wave voltammetry. The electrochemical results obtained were used for the development of the HPLC-ED method. The HPLC-ED system used consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. Isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate of 0.4 mL/min. The full scan of multi-channel coulometric detection was tested and an optimal potential at 450 mV was chosen for our purposes. The calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The relative standard deviation (R.S.D., n = 5) was 2.5 %. The detection limits for daidzein and genistein were 480 and 394 pg/mL (1.8 and 1.5 nM), respectively. Isoflavones extracted from soybean products (farina, meat, milk) by the pressurised solvent extraction procedure and isoflavones contained in human urine were determined by this HPLC-ED method.
ER -
KLEJDUS, Bořivoj, Jitka PETRLOVÁ, David POTĚŠIL, Libuše TRNKOVÁ, Radka MIKELOVÁ, Josef ZEHNÁLEK, René KIZEK and Vlastimil KUBÁŇ. Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED). In \textit{Book of Abstracts - 55th Annual Meeting of the ISE}. 1st ed. Thessaloniki, Greece: International Society of Electrochemistry, 2004, p.~433 - 433.
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