Cell growth and division processes are differentially sensitive
to cadmium in Scenedesmus quadricauda

J 2003

Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda

BIŠOVÁ, Kateřina, Jana HENDRYCHOVÁ, Vladislav CEPÁK and Vilém ZACHLEDER

Basic information

Original name

Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda

Name in Czech

Růstové a reprodukční procesy v buňce řasy Scenedesmus quadricauda jsou různě citlivé ke kadmiu

Authors

BIŠOVÁ, Kateřina (203 Czech Republic), Jana HENDRYCHOVÁ (203 Czech Republic), Vladislav CEPÁK (203 Czech Republic, guarantor) and Vilém ZACHLEDER (203 Czech Republic)

Edition

Folia Microbiologica, ČR, Mikrobiologický ústav AV ČR Praha, 2003, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.857

RIV identification code

RIV/00216224:14310/03:00012033

Organization unit

Faculty of Science

UT WoS

000189360400015

Keywords in English

toxicity; Scenedesmus; growth; H1 kinases

Abstract

ORIG CZ

V originále

The effect of cadmium on growth processes (the accumulation of RNA, proteins and cell volume), cell cycle reproductive events (DNA replication, mitosis, protoplast fission and daughter?cell formation) and the regulatory activity of histone H1 kinases were monitored in synchronized cultures of the chlorococcal alga Scenedesmus quadricauda (Turp.) Bréb. Distinct dosage?dependent inhibitory effects of cadmium ions were found in individual growth and reproductive processes. At concentrations of about 60 mmol/L CdCl2, the growth processes were slowed after about half of the cell cycle but the cells grew to the same or larger size than did the untreated cells. At higher concentrations of CdCl2, the growth became progressively inhibited, being completely blocked at concentrations above 240 mmol/L CdCl2. Total RNA accumulation was the most sensitive growth process. Each of the reproductive events was a target for cadmium ions with increasing sensitivity in the following order: DNA replication, mitosis, protoplast fission, and daughter?cell formation. Throughout the entire experiment, the activity of "mitosis?specific" histone H1 kinases was negligible in the cadmium (60 mmol/L CdCl2) treated cultures, whilst that of the control culture varied, peaking just prior to nuclear divisions. The activity of "growth?associated" histone H1 kinases was not affected by cadmium ions. No effect was found if cadmium was present during the pre?commitment period. The longer the period in the presence of cadmium chloride, the more it caused the inhibition of reproductive events.

In Czech

viz angl. verze

Links

GA204/99/1235, research and development project

Name: Vliv těžkých kovů na cytoskelet řas

Investor: Czech Science Foundation, The effect of heavy metals on cytoskeleton of algae

Citovat

BIŠOVÁ, Kateřina, Jana HENDRYCHOVÁ, Vladislav CEPÁK and Vilém ZACHLEDER. Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda. Folia Microbiologica. ČR: Mikrobiologický ústav AV ČR Praha, 2003, vol. 48, No 6, p. 805-816. ISSN 0015-5632.

@article{568567,
   author = {Bišová, Kateřina and Hendrychová, Jana and Cepák, Vladislav and Zachleder, Vilém},
   article_location = {ČR},
   article_number = {6},
   keywords = {toxicity; Scenedesmus; growth; H1 kinases},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda},
   volume = {48},
   year = {2003}
}

TY  - JOUR
ID  - 568567
AU  - Bišová, Kateřina - Hendrychová, Jana - Cepák, Vladislav - Zachleder, Vilém
PY  - 2003
TI  - Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda
JF  - Folia Microbiologica
VL  - 48
IS  - 6
SP  - 805-816
EP  - 805-816
PB  - Mikrobiologický ústav AV ČR Praha
SN  - 00155632
KW  - toxicity
KW  - Scenedesmus
KW  - growth
KW  - H1 kinases
N2  - The effect of cadmium on growth processes (the accumulation of RNA, proteins and cell volume), cell cycle reproductive events (DNA replication, mitosis, protoplast fission and daughter?cell formation) and the regulatory activity of histone H1 kinases were monitored in synchronized cultures of the chlorococcal alga Scenedesmus quadricauda (Turp.) Bréb. Distinct dosage?dependent inhibitory effects of cadmium ions were found in individual growth and reproductive processes. At concentrations of about 60 mmol/L CdCl2, the growth processes were slowed after about half of the cell cycle but the cells grew to the same or larger size than did the untreated cells. At higher concentrations of CdCl2, the growth became progressively inhibited, being completely blocked at concentrations above 240 mmol/L CdCl2. Total RNA accumulation was the most sensitive growth process. Each of the reproductive events was a target for cadmium ions with increasing sensitivity in the following order: DNA replication, mitosis, protoplast fission, and daughter?cell formation. Throughout the entire experiment, the activity of "mitosis?specific" histone H1 kinases was negligible in the cadmium (60 mmol/L CdCl2) treated cultures, whilst that of the control culture varied, peaking just prior to nuclear divisions. The activity of "growth?associated" histone H1 kinases was not affected by cadmium ions. No effect was found if cadmium was present during the pre?commitment period. The longer the period in the presence of cadmium chloride, the more it caused the inhibition of reproductive events.
ER  -

BIŠOVÁ, Kateřina, Jana HENDRYCHOVÁ, Vladislav CEPÁK and Vilém ZACHLEDER. Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda. \textit{Folia Microbiologica}. ČR: Mikrobiologický ústav AV ČR Praha, 2003, vol.~48, No~6, p.~805-816. ISSN~0015-5632.

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