The effect of nalidixic acid on growth and reproductive events
in nucleocytosolic and chloroplast compartments in the alga
Scenedesmus quadricauda

J 2004

The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda

ZACHLEDER, Vilém, Shigeki KAWANO, Vladislav CEPÁK and Tsuneoshi KUROIWA

Basic information

Original name

The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda

Name in Czech

Vliv nalidixové kyseliny na růst a reprodukční děje v nukleocytoplasmě a chloroplastu u řasy Scenedesmus quadricauda

Authors

ZACHLEDER, Vilém (203 Czech Republic, guarantor), Shigeki KAWANO (392 Japan), Vladislav CEPÁK (203 Czech Republic) and Tsuneoshi KUROIWA (392 Japan)

Edition

Folia Microbiologica, Praha, Mikrobiologický ústav AV ČR, 2004, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.034

RIV identification code

RIV/00216224:14330/04:00012034

Organization unit

Faculty of Informatics

UT WoS

000224416700011

Keywords in English

cell cycle; regulation; alga; Scenedesmus

Abstract

ORIG CZ

V originále

The courses of rRNA accumulation, DNA replication, and nuclear division were followed both in the chloroplast and the nucleocytosolic compartments during the cell cycle in synchronized populations of the chlorococcal alga Scenedesmus quadricauda. Control and nalidixic acid treated cultures were compared. Nalidixic acid (150 mg/L) was added either at the beginning of the cell cycle or consecutively during the cell cycle to subcultures transferred into the dark. If the inhibitor was applied at the beginning of the cell cycle, chloroplast DNA did not replicate and nucleoids did not divide. Chloroplast division, however, was coordinated in a timely fashion with cytokinesis even under conditions of blocked chloroplast DNA replication. While the growth rate was slowed down, the courses of reproductive processes in the nucleocytosolic compartment were not affected and their timing and the number of rounds were coordinated with growth rate as in the control culture. The rate of cytosolic rRNA synthesis was slowed but no apparent effect was seen on the amount of rRNA that accumulated during the cell cycle. In contrast, lower levels of chloroplast rRNA were found at the end of the cell cycle compared with the control culture. Experiments in which cells were transferred to the dark during the cell cycle showed that the inhibitor affected none of the reproductive events in the nucleocytosolic compartment. In the chloroplast compartment, DNA replication was inhibited in inhibitor treated cultures, but was unaffected in controls. The chloroplast nucleoids themselves divided even in the presence of inhibitor, reducing their DNA content to a level which corresponded to that in freshly formed control daughter cells.

In Czech

viz angl. verze

Links

GA204/02/1438, research and development project

Name: Změny v aktivitě komplexů cyklin dependentních kinas a ve fosforylaci jejich cílových bílkovin ve funkčním vztahu k dějům buněčného cyklu řas

Citovat

ZACHLEDER, Vilém, Shigeki KAWANO, Vladislav CEPÁK and Tsuneoshi KUROIWA. The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda. Folia Microbiologica. Praha: Mikrobiologický ústav AV ČR, 2004, vol. 49, No 4, p. 441-451. ISSN 0015-5632.

@article{568594,
   author = {Zachleder, Vilém and Kawano, Shigeki and Cepák, Vladislav and Kuroiwa, Tsuneoshi},
   article_location = {Praha},
   article_number = {4},
   keywords = {cell cycle; regulation; alga; Scenedesmus},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda},
   volume = {49},
   year = {2004}
}

TY  - JOUR
ID  - 568594
AU  - Zachleder, Vilém - Kawano, Shigeki - Cepák, Vladislav - Kuroiwa, Tsuneoshi
PY  - 2004
TI  - The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda
JF  - Folia Microbiologica
VL  - 49
IS  - 4
SP  - 441-451
EP  - 441-451
PB  - Mikrobiologický ústav AV ČR
SN  - 00155632
KW  - cell cycle
KW  - regulation
KW  - alga
KW  - Scenedesmus
N2  - The courses of rRNA accumulation, DNA replication, and nuclear division were followed both in the chloroplast and the nucleocytosolic compartments during the cell cycle in synchronized populations of the chlorococcal alga Scenedesmus quadricauda. Control and nalidixic acid treated cultures were compared. Nalidixic acid (150 mg/L) was added either at the beginning of the cell cycle or consecutively during the cell cycle to subcultures transferred into the dark. If the inhibitor was applied at the beginning of the cell cycle, chloroplast DNA did not replicate and nucleoids did not divide. Chloroplast division, however, was coordinated in a timely fashion with cytokinesis even under conditions of blocked chloroplast DNA replication. While the growth rate was slowed down, the courses of reproductive processes in the nucleocytosolic compartment were not affected and their timing and the number of rounds were coordinated with growth rate as in the control culture. The rate of cytosolic rRNA synthesis was slowed but no apparent effect was seen on the amount of rRNA that accumulated during the cell cycle. In contrast, lower levels of chloroplast rRNA were found at the end of the cell cycle compared with the control culture. Experiments in which cells were transferred to the dark during the cell cycle showed that the inhibitor affected none of the reproductive events in the nucleocytosolic compartment. In the chloroplast compartment, DNA replication was inhibited in inhibitor treated cultures, but was unaffected in controls. The chloroplast nucleoids themselves divided even in the presence of inhibitor, reducing their DNA content to a level which corresponded to that in freshly formed control daughter cells.
ER  -

ZACHLEDER, Vilém, Shigeki KAWANO, Vladislav CEPÁK and Tsuneoshi KUROIWA. The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda. \textit{Folia Microbiologica}. Praha: Mikrobiologický ústav AV ČR, 2004, vol.~49, No~4, p.~441-451. ISSN~0015-5632.

Detailed Information on Publication Record