ŽÁKOVSKÁ, Alena, Petra NEJEDLÁ, Alena HOLÍKOVÁ and Miloš DENDIS. Determination of tick positivity for pathogenic Borrelia burgdorferi by using PCR-RFLP method. Scripta medica. 2004, vol. 77, No 2, p. 100. ISSN 1211-3395.
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Basic information
Original name Determination of tick positivity for pathogenic Borrelia burgdorferi by using PCR-RFLP method
Name in Czech Stanovení pozitivity klíšťat na patogenní Borrelia burgdorferi pomocí PCR-RFLP metody
Authors ŽÁKOVSKÁ, Alena (203 Czech Republic, guarantor), Petra NEJEDLÁ (203 Czech Republic), Alena HOLÍKOVÁ (203 Czech Republic) and Miloš DENDIS (203 Czech Republic).
Edition Scripta medica, 2004, 1211-3395.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/04:00021313
Organization unit Faculty of Science
Keywords in English tick; Borrelia burgdorferi;Lyme disease; DFM; PCR; PCR-RFLP
Tags Borrelia burgdorferi, DFM, Lyme disease, PCR, PCR-RFLP, tick
Changed by Changed by: doc. RNDr. Alena Žákovská, Ph.D., učo 417. Changed: 22/7/2008 21:00.
Abstract
The aim of the study was the monitoring of the presence of Borrelia burgdorferi in ticks Ixodes ricinus in the Pisárky park, Brno in 1996-2001. Ticks were analysed by DFM and next by the PCR method. The samples with a greater mass of borrelia DNA were analysed by the PCR-RFLP method and transfered into BSK-H medium and cultivated.
Abstract (in Czech)
Cíl našeho studia bylo monitorování přítomnosti Borrelia burgdorferi v klíšťatech Ixodes ricinus v parku Pisárky v Brně v letech 1996-2001. Klíšťata byla vyšetřována metodou DFM a po té pomocí PCR. Vzorky s větším obsahem borreliové DNA byly detekovány metodou PCR-RFLP, přeneseny do BSK-H media a kultivovány.
Links
MSM 143100010, plan (intention)Name: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.
Investor: Ministry of Education, Youth and Sports of the CR, Spatiotemporal biodiversity dynamics in ecosystems of Central Europe
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