| SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS a Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. Analytica Chimica Acta. Amsterdam: Elsevier Science Publishers, 2005, roč. 528, č. 1, s. 9-19. ISSN 0003-2670. |
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@article{570041,
author = {Solná, Renáta and Dock, Eva and Christenson, Alexander and WinterandNielsen, Margrethe and Carlsson, Christine and Emnéus, Jenny and Ruzgas, Tautgirdas and Skládal, Petr},
article_location = {Amsterdam},
article_number = {1},
keywords = {amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases},
volume = {528},
year = {2005}
}
TY - JOUR
ID - 570041
AU - Solná, Renáta - Dock, Eva - Christenson, Alexander - Winter-Nielsen, Margrethe - Carlsson, Christine - Emnéus, Jenny - Ruzgas, Tautgirdas - Skládal, Petr
PY - 2005
TI - Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases
JF - Analytica Chimica Acta
VL - 528
IS - 1
SP - 9-19
EP - 9-19
PB - Elsevier Science Publishers
SN - 00032670
KW - amperometric multienzyme sensor
KW - steady state system
KW - cholinesterase
KW - oxidoreductase
KW - phenoks
KW - pesticides
KW - wastewater
KW - multivariate-analysis
N2 - Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).
ER -
SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS a Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science Publishers, 2005, roč.~528, č.~1, s.~9-19. ISSN~0003-2670.
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