SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS and Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. Analytica Chimica Acta. Amsterdam: Elsevier Science Publishers, 2005, vol. 528, No 1, p. 9-19. ISSN 0003-2670.
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Basic information
Original name Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases
Name in Czech Amperometrické sítotisková biosensorová pole s koimobilisovanými oxidoreduktasami a cholinesterasami
Authors SOLNÁ, Renáta (203 Czech Republic), Eva DOCK (752 Sweden), Alexander CHRISTENSON (752 Sweden), Margrethe WINTER-NIELSEN (208 Denmark), Christine CARLSSON (752 Sweden), Jenny EMNÉUS (752 Sweden), Tautgirdas RUZGAS (752 Sweden) and Petr SKLÁDAL (203 Czech Republic, guarantor).
Edition Analytica Chimica Acta, Amsterdam, Elsevier Science Publishers, 2005, 0003-2670.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.760
RIV identification code RIV/00216224:14310/05:00013673
Organization unit Faculty of Science
UT WoS 000226455600002
Keywords in English amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis
Tags amperometric multienzyme sensor, Cholinesterase, multivariate-analysis, oxidoreductase, pesticides, phenoks, steady state system, wastewater
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 7/6/2005 16:04.
Abstract
Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).
Abstract (in Czech)
Amperometrické sítotiskové multielektrodové biosensory byly připraveny použitím cholinesteras (AChE a BChE), tyrosinasy, celobiosa dehydrogenasy a peroxidas (křenová a sojová) na jediném sensoru. Byly optimalisovány podmínky měření. Testované vzorky odpadních vod pak byly klasifikovány pomocí chemometrické multivariační analysy.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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