Amperometric screen-printed biosensor arrays with
co-immobilised oxidoreductases and cholinesterases

J 2005

Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON et. al.

Basic information

Original name

Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

Name in Czech

Amperometrické sítotisková biosensorová pole s koimobilisovanými oxidoreduktasami a cholinesterasami

Authors

SOLNÁ, Renáta (203 Czech Republic), Eva DOCK (752 Sweden), Alexander CHRISTENSON (752 Sweden), Margrethe WINTER-NIELSEN (208 Denmark), Christine CARLSSON (752 Sweden), Jenny EMNÉUS (752 Sweden), Tautgirdas RUZGAS (752 Sweden) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Analytica Chimica Acta, Amsterdam, Elsevier Science Publishers, 2005, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.760

RIV identification code

RIV/00216224:14310/05:00013673

Organization unit

Faculty of Science

UT WoS

000226455600002

Keywords in English

amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis

Abstract

ORIG CZ

V originále

Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).

In Czech

Amperometrické sítotiskové multielektrodové biosensory byly připraveny použitím cholinesteras (AChE a BChE), tyrosinasy, celobiosa dehydrogenasy a peroxidas (křenová a sojová) na jediném sensoru. Byly optimalisovány podmínky měření. Testované vzorky odpadních vod pak byly klasifikovány pomocí chemometrické multivariační analysy.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS and Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. Analytica Chimica Acta. Amsterdam: Elsevier Science Publishers, 2005, vol. 528, No 1, p. 9-19. ISSN 0003-2670.

@article{570041,
   author = {Solná, Renáta and Dock, Eva and Christenson, Alexander and WinterandNielsen, Margrethe and Carlsson, Christine and Emnéus, Jenny and Ruzgas, Tautgirdas and Skládal, Petr},
   article_location = {Amsterdam},
   article_number = {1},
   keywords = {amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases},
   volume = {528},
   year = {2005}
}

TY  - JOUR
ID  - 570041
AU  - Solná, Renáta - Dock, Eva - Christenson, Alexander - Winter-Nielsen, Margrethe - Carlsson, Christine - Emnéus, Jenny - Ruzgas, Tautgirdas - Skládal, Petr
PY  - 2005
TI  - Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases
JF  - Analytica Chimica Acta
VL  - 528
IS  - 1
SP  - 9-19
EP  - 9-19
PB  - Elsevier Science Publishers
SN  - 00032670
KW  - amperometric multienzyme sensor
KW  - steady state system
KW  - cholinesterase
KW  - oxidoreductase
KW  - phenoks
KW  - pesticides
KW  - wastewater
KW  - multivariate-analysis
N2  - Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).
ER  -

SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS and Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science Publishers, 2005, vol.~528, No~1, p.~9-19. ISSN~0003-2670.

Detailed Information on Publication Record