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@inproceedings{570240, author = {Váňa, Petr and Zahradníčková, Eva and Povolná, Karolína and Konečná, Hana and Zdráhal, Zbyněk and Šmarda, Jan}, address = {Bratislava}, booktitle = {Študentská vedecká konferencia}, keywords = {Proteome; v-myb; differentiation; mass spectrometry; two-dimensional electrohoresis}, language = {eng}, location = {Bratislava}, isbn = {80-88870-46-1}, pages = {148-148}, publisher = {Univerzita Komenského v Bratislave}, title = {Versatility of proteome during terminal differentiation of v-myb-transformed monoblasts}, year = {2005} }
TY - JOUR ID - 570240 AU - Váňa, Petr - Zahradníčková, Eva - Povolná, Karolína - Konečná, Hana - Zdráhal, Zbyněk - Šmarda, Jan PY - 2005 TI - Versatility of proteome during terminal differentiation of v-myb-transformed monoblasts PB - Univerzita Komenského v Bratislave CY - Bratislava SN - 8088870461 KW - Proteome KW - v-myb KW - differentiation KW - mass spectrometry KW - two-dimensional electrohoresis N2 - In order to investigate the versatility of proteom of leukemic cells undergoing terminal differentiation, we performed two-dimensional electrophoresis of proteins extracted from BM2 cells induced with phorbol ester TPA, trichostatin A and retinoic acid for various time periods and compared it with two-dimensional electrophoretogram of untreated control cells. Some of the spots of differentially expressed proteins were digested with trypsin and identified by MALDI-TOF mass spectrometry as heat shock protein 70, protein-glutamine glutamyltransferase 4, prostaglandin-D-synthase, EMS1, nucleobindin 2 precursor (DNA-binding protein NEFA), actin, ARP2/3 protein complex subunit 3 and chaperonin Hsp60. Biological significance of versatility of these proteins in differentiating BM2 cells is currently investigated. ER -
VÁŇA, Petr, Eva ZAHRADNÍČKOVÁ, Karolína POVOLNÁ, Hana KONEČNÁ, Zbyněk ZDRÁHAL and Jan ŠMARDA. Versatility of proteome during terminal differentiation of v-myb-transformed monoblasts. In \textit{Študentská vedecká konferencia}. Bratislava: Univerzita Komenského v Bratislave, 2005, p.~148. ISBN~80-88870-46-1.
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