Construction of Cryptogein Mutants, a Proteinaceous Elicitor
from Phytophthora, with Altered Abilities To Induce a Defense
Reaction in Tobacco Cells

J 2005

Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells

LOCHMAN, Jan, Tomáš KAŠPAROVSKÝ, Jiří DAMBORSKÝ, Hanan OSMAN, MIchel PONCHET et. al.

Basic information

Original name

Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells

Name in Czech

Konstrukce mutantu kryptogeinu, elicitoru houby Phytophthora, s modifikovanými vlastnostmi idnukce obranné reakce u bunek tabaku

Authors

LOCHMAN, Jan (203 Czech Republic), Tomáš KAŠPAROVSKÝ (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic), Hanan OSMAN (250 France), MIchel PONCHET (250 France), Vladimír MIKEŠ (203 Czech Republic, guarantor) and Radka CHALOUPKOVÁ (203 Czech Republic)

Edition

Biochemistry, USA, 2005, 0006-2960

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.848

RIV identification code

RIV/00216224:14310/05:00012448

Organization unit

Faculty of Science

UT WoS

000228731700019

Keywords in English

Elicitor; Phytophthora; defense

Abstract

ORIG CZ

V originále

We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their ability to bind sterols but not fatty acids. Although the cryptogein-sterol complex was suggested to be a form triggering a defense reaction in tobacco, some proteins unable to bind sterols induced the synthesis of active oxygen species and pH changes. The modeling experiments showed that conformational changes after the introduction of bulky residues into the loop of cryptogein resemble those induced by sterol binding. These changes may be necessary for the ability to trigger the early events by elicitins. However, the ability to stimulate necrosis in suspension tobacco cells and the expression of defense proteins in tobacco plants were linked neither to the lipid binding capacity nor to the capacity to provoke the early events. On the basis of these experiments and previous results, we propose that elicitins could stimulate two signal pathways. The first one induces necroses and the expression of pathogen-related proteins, includes tyrosine protein kinases and mitogen-activated protein kinases, and depends on the overall structure and charge distribution. The second type of interaction is mediated by phospholipase C and protein kinase C. It triggers the synthesis of active oxygen species and pH changes. This interaction depends on the ability of elicitins to bind sterols.

In Czech

Konstrukce mutantu kryptogeinu, elicitoru houby Phytophthora

Links

GA522/02/0925, research and development project

Name: Modelování struktury a interakce bílkovinných elicitorů houby Phytophthora

Investor: Czech Science Foundation, Modeling of the structure and interaction of elicitors of the fungus Phytophthora

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

LOCHMAN, Jan, Tomáš KAŠPAROVSKÝ, Jiří DAMBORSKÝ, Hanan OSMAN, MIchel PONCHET, Vladimír MIKEŠ and Radka CHALOUPKOVÁ. Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells. Biochemistry. USA, 2005, vol. 44, No 17, p. 6565-6577. ISSN 0006-2960.

@article{570714,
   author = {Lochman, Jan and Kašparovský, Tomáš and Damborský, Jiří and Osman, Hanan and Ponchet, MIchel and Mikeš, Vladimír and Chaloupková, Radka},
   article_number = {17},
   keywords = {Elicitor; Phytophthora; defense},
   language = {eng},
   issn = {0006-2960},
   journal = {Biochemistry},
   title = {Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells},
   volume = {44},
   year = {2005}
}

TY  - JOUR
ID  - 570714
AU  - Lochman, Jan - Kašparovský, Tomáš - Damborský, Jiří - Osman, Hanan - Ponchet, MIchel - Mikeš, Vladimír - Chaloupková, Radka
PY  - 2005
TI  - Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells
JF  - Biochemistry
VL  - 44
IS  - 17
SP  - 6565
EP  - 6565
PB  - USA
SN  - 00062960
KW  - Elicitor
KW  - Phytophthora
KW  - defense
N2  - We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their ability to bind sterols but not fatty acids. Although the cryptogein-sterol complex was suggested to be a form triggering a defense reaction in tobacco, some proteins unable to bind sterols induced the synthesis of active oxygen species and pH changes. The modeling experiments showed that conformational changes after the introduction of bulky residues into the loop of cryptogein resemble those induced by sterol binding. These changes may be necessary for the ability to trigger the early events by elicitins. However, the ability to stimulate necrosis in suspension tobacco cells and the expression of defense proteins in tobacco plants were linked neither to the lipid binding capacity nor to the capacity to provoke the early events. On the basis of these experiments and previous results, we propose that elicitins could stimulate two signal pathways. The first one induces necroses and the expression of pathogen-related proteins, includes tyrosine protein kinases and mitogen-activated protein kinases, and depends on the overall structure and charge distribution. The second type of interaction is mediated by phospholipase C and protein kinase C. It triggers the synthesis of active oxygen species and pH changes. This interaction depends on the ability of elicitins to bind sterols.
ER  -

LOCHMAN, Jan, Tomáš KAŠPAROVSKÝ, Jiří DAMBORSKÝ, Hanan OSMAN, MIchel PONCHET, Vladimír MIKEŠ and Radka CHALOUPKOVÁ. Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells. \textit{Biochemistry}. USA, 2005, vol.~44, No~17, p.~6565-6577. ISSN~0006-2960.

Detailed Information on Publication Record