Characterisation of Bifidobacterium and Lactobacillus strains in infant faeces samples
| ŠPANOVÁ, Alena, Bohuslav RITTICH, Lenka KLESNILOVÁ and Tereza ŠKAPOVÁ. Characterisation of Bifidobacterium and Lactobacillus strains in infant faeces samples. In BioMicroWorld-2005. Badajoz: Formatex Research Center, 2005, p. 1084-1084. |
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| Basic information | |
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| Original name | Characterisation of Bifidobacterium and Lactobacillus strains in infant faeces samples |
| Name in Czech | Charakterizace bakteriálních kmenů rodu Bifidobacterium and Lactobacillus ve vzorcích dětské stolice |
| Authors | ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Bohuslav RITTICH (203 Czech Republic), Lenka KLESNILOVÁ (203 Czech Republic) and Tereza ŠKAPOVÁ (203 Czech Republic). |
| Edition | Badajoz, BioMicroWorld-2005, p. 1084-1084, 1 pp. 2005. |
| Publisher | Formatex Research Center |
| Other information | |
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| Original language | English |
| Type of outcome | Proceedings paper |
| Field of Study | 10600 1.6 Biological sciences |
| Country of publisher | Spain |
| Confidentiality degree | is not subject to a state or trade secret |
| RIV identification code | RIV/00216224:14310/05:00015263 |
| Organization unit | Faculty of Science |
| Keywords in English | Bifidobacterium; Lactobacillus; infant faeces; PCR; ARDRA; RAPD |
| Tags | ARDRA, Bifidobacterium, infant faeces, Lactobacillus, PCR, RAPD |
| Changed by | Changed by: doc. Ing. Bohuslav Rittich, CSc., učo 1639. Changed: 5/12/2005 12:14. |
| Abstract |
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| In the present study genus-specific and species-specific PCR primers (targeted on a highly conserved 16S rDNA region) were used to identify Bifidobacterium and Lactobacillus strains isolated from infant faecal samples. Bifidobacteria were differentiated at the species level by a restriction fragment length polymorphism analysis using the enzymes BamHI, Sau3AI, and TaqI (amplified ribosomal DNA restriction analysis ARDRA). Bifidobacteria and lactobacilli were also differentiated using RAPD analysis. Altogether 40 strains of Bifidobacteria and 20 strains of Lactobacillus identified in the collected infant faeces were analysed. |
| Abstract (in Czech) |
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| Rodově a druhově specifické primery (odvozené z vysoce konzervativní 16S rDNA oblasti)byly použity k identifikaci kmenů rodu bifidobacterium a Lactobacillus, které byly izolovány z dětské stolice. Bifidobakterie byly rozlišeny na úroveň druhů pomocí restrikční analýzy PCR produktů použitím restrikčních endonukleas BamHI, Sau3AI, a TaqI (ARDRA). Bifidobakterie a lactobacily byly rovněž rozlišeny pomocí RAPD analýzy. Celkem bylo analyzováno 40 kmenů rodu Bifidobacterium a 20 kmenů rodu Lactobacillus. |
| Links | |
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| QF3169, research and development project | Name: Využití postupů na bázi molekulární genetiky při získávání nových kmenů baktérií s probiotickými vlastnostmi s cílem rozšíření specifických genetických zdrojů pro výrobu funkčních potravin |
| Investor: Ministry of Agriculture of the CR, The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods | |
| 1G46045, research and development project | Name: Molekulárně biologická analýza tuzemského fondu produkčních kmenů baktérií mléčného kvašení. |
| Investor: Ministry of Agriculture of the CR, Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods | |
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