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@inproceedings{571021, author = {Matejková, Michaela and Padrta, Petr and Fiala, Radovan and Sklenář, Vladimír}, address = {Brno}, booktitle = {19th NMR Valtice : Central European NMR Discussion Groups}, keywords = {NMR;DNA;structure}, language = {eng}, location = {Brno}, isbn = {80-210-3352-5}, pages = {29-29}, publisher = {Masarykova univerzita}, title = {NMR Distance Restraints At Low Temperatures : An Application to DNA Hairpin.}, year = {2004} }
TY - JOUR ID - 571021 AU - Matejková, Michaela - Padrta, Petr - Fiala, Radovan - Sklenář, Vladimír PY - 2004 TI - NMR Distance Restraints At Low Temperatures : An Application to DNA Hairpin. PB - Masarykova univerzita CY - Brno SN - 8021033525 KW - NMR;DNA;structure N2 - Short DNA hairpins play a significant role in a number of biological processes. The most interesting feature of the oligonucleotides with a general sequence d(GCGNAGC) (N=A,G,C,T) is their extraordinary stability represented by high melting temperatures, polyacrylamide gel mobility, and resistance against nucleases. Detailed knowledge of these structures helps understand their unique behavior. One of these fragments, the d(GCGAAGC) structure has been solved previously by NMR spectroscopy using the NOE-derived distances, torsion angles, and residual dipolar couplings [1]. The aim of the present study is a new structure calculation of d(GCGAAGC) based on NOE data measured at temperatures below 273 K when the intramolecular motions and the chemical exchange of the amino protons are inhibited. We focused here on a comparison of number of restraints derived from the NOESY spectra at 268K and 303K, paying our attention to amino and imino connectivities within a molecule. The approach used for the d(GCGAAGC) structure calculation should also be used for the calculation of the corresponding r(GCGAAGC) hairpin structure which is much less stable. ER -
MATEJKOVÁ, Michaela, Petr PADRTA, Radovan FIALA and Vladimír SKLENÁŘ. NMR Distance Restraints At Low Temperatures : An Application to DNA Hairpin. In \textit{19th NMR Valtice : Central European NMR Discussion Groups}. Brno: Masarykova univerzita, 2004, p.~29-29. ISBN~80-210-3352-5.
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