| HAVLIŠ, Jan, Tobias OELSCHLAEGEL, Martin SCHWICKART, Joao MATOS, Aliona BOGDANOVA, Alain CAMASSES, Andrej SHEVCHENKO and Wolfgang ZACHARIAE. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. United States of America: Cell Press, Cambridge, MA, USA, vol. 120, No 6, p. 773-788, 15 pp. ISSN 0092-8674. 2005. |
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@article{571157,
author = {Havliš, Jan and Oelschlaegel, Tobias and Schwickart, Martin and Matos, Joao and Bogdanova, Aliona and Camasses, Alain and Shevchenko, Andrej and Zachariae, Wolfgang},
article_location = {United States of America},
article_number = {6},
keywords = {anaphase-promoting complex; chromosome segregation; fission yeast; centromeric cohesion; spore formation; protein family; kinase cdc5; cleavage; mitosis; proteolysis},
language = {eng},
issn = {0092-8674},
journal = {Cell},
title = {The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1},
volume = {120},
year = {2005}
}
TY - JOUR
ID - 571157
AU - Havliš, Jan - Oelschlaegel, Tobias - Schwickart, Martin - Matos, Joao - Bogdanova, Aliona - Camasses, Alain - Shevchenko, Andrej - Zachariae, Wolfgang
PY - 2005
TI - The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1
JF - Cell
VL - 120
IS - 6
SP - 773-788
EP - 773-788
PB - Cell Press, Cambridge, MA, USA
SN - 00928674
KW - anaphase-promoting complex
KW - chromosome segregation
KW - fission yeast
KW - centromeric cohesion
KW - spore formation
KW - protein family
KW - kinase cdc5
KW - cleavage
KW - mitosis
KW - proteolysis
N2 - Cohesion established between sister chromatids during pre-meiotic DNA replication mediates two rounds of chromosome segregation. The first division is preceded by an extended prophase wherein homologous chromosomes undergo recombination. The persistence of cohesion during prophase is essential for recombination and both meiotic divisions. Here we show that Mnd2, a subunit of the anaphase-promoting complex (APC/C) from budding yeast, is essential to prevent premature destruction of cohesion in meiosis. During S- and prophase, Mnd2 prevents activation of the APC/C by a melosis-specific activator called Ama1. In cells lacking Mnd2 the APC/C-Ama1 enzyme triggers degradation of Pds1, which causes premature sister chromatid separation due to unrestrained separase activity. In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C.
ER -
HAVLIŠ, Jan, Tobias OELSCHLAEGEL, Martin SCHWICKART, Joao MATOS, Aliona BOGDANOVA, Alain CAMASSES, Andrej SHEVCHENKO and Wolfgang ZACHARIAE. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. \textit{Cell}. United States of America: Cell Press, Cambridge, MA, USA, vol.~120, No~6, p.~773-788, 15 pp. ISSN~0092-8674. 2005.
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